Jöhren O, Saavedra J M
Section on Pharmacology, National Institute of Mental Health, Bethesda, Maryland 20892, USA.
Am J Physiol. 1996 Jul;271(1 Pt 1):E104-12. doi: 10.1152/ajpendo.1996.271.1.E104.
The gene expression of angiotensin II receptor subtypes AT1A and AT1B was localized in the forebrain of 2-wk-old rats by in situ hybridization histochemistry and compared with [125I]Sar1-angiotensin II binding patterns. AT1A receptor mRNA was expressed in circumventricular organs, in hypothalamic nuclei like the paraventricular nucleus, in the lateral olfactory tract, in the basolateral amygdaloid and anterior olfactory nuclei, and in the piriform cortex. No AT1B receptor mRNA was detected in these areas. AT1A and AT1B receptor mRNA was detected in the hippocampus, cingulate cortex, and choroid plexus. No forebrain area studied expressed AT1B receptor mRNA exclusively. Most often, a good match for receptor mRNA and binding was found. In some areas, apparent mismatches suggested receptor formation elsewhere (median eminence) or receptor presence in local neuronal circuits (hippocampus, cingulate, and piriform cortex). Our results support the hypothesis that AT1A receptors are involved in the classical central functions of angiotensin II. Both AT1A and AT1B receptors may play roles in cortical and limbic system function, particularly early in development.
通过原位杂交组织化学方法，对2周龄大鼠前脑内血管紧张素II受体亚型AT1A和AT1B的基因表达进行定位，并与[125I]Sar1-血管紧张素II结合模式进行比较。AT1A受体mRNA在室周器官、下丘脑室旁核等下丘脑核团、外侧嗅束、基底外侧杏仁核和前嗅核以及梨状皮质中表达。在这些区域未检测到AT1B受体mRNA。在海马、扣带回皮质和脉络丛中检测到了AT1A和AT1B受体mRNA。所研究的前脑区域均未仅表达AT1B受体mRNA。大多数情况下，受体mRNA与结合情况匹配良好。在一些区域，明显的不匹配提示受体在其他部位（正中隆起）形成或在局部神经回路（海马、扣带回和梨状皮质）中存在。我们的结果支持以下假说：AT1A受体参与血管紧张素II的经典中枢功能。AT1A和AT1B受体可能在皮质和边缘系统功能中发挥作用，尤其是在发育早期。
