Tan C K, Hastings P J
Mol Gen Genet. 1977 Apr 29;152(3):311-7. doi: 10.1007/BF00693085.
In strain 137F of Chlamydomonas reinhardi, the zygospores undergo one round of nuclear DNA replication followed by three divisions to produce octospores. The third division without replication has been interpreted by Sueoka et al. (1967, 1969) to mean that the gametes and vegetative cells have at least binemic chromosomes. We have repeated their experiments using the same strain. However, the meiotic products were inviable--unable to undergo postmeiotic vegetative growth, DNA replication or division. On the other hand, using a variant of strain 137C which also has three divisions during germination we have shown that meiosis is normal. Zygospores from this strain undergo two rounds of nuclear DNA replication prior to the formation of octospores. These meiotic products are viable and capable of postmeiotic vegetative growth, replication and division. Since the third division without DNA replication subsequent to the two meiotic divisions leads to inviable products, and the strain which has viable products after three divisions does not lack the additional replication, meiosis in Chlamydomonas reinhardi provides no evidence of a bineme chromosome structure.
在莱茵衣藻的137F品系中，接合孢子经历一轮核DNA复制，随后进行三次分裂以产生八核孢子。第三次无复制的分裂被末冈等人（1967年、1969年）解释为意味着配子和营养细胞至少有双联体染色体。我们使用同一品系重复了他们的实验。然而，减数分裂产物是不可育的——无法进行减数分裂后的营养生长、DNA复制或分裂。另一方面，使用137C品系的一个变种，该变种在萌发过程中也有三次分裂，我们已经表明减数分裂是正常的。来自该品系的接合孢子在形成八核孢子之前经历两轮核DNA复制。这些减数分裂产物是可育的，并且能够进行减数分裂后的营养生长、复制和分裂。由于在两次减数分裂后没有DNA复制的第三次分裂导致不可育产物，并且在三次分裂后有可育产物的品系并不缺少额外的复制，莱茵衣藻中的减数分裂没有提供双联体染色体结构的证据。
