减数分裂期间的染色体复制：减数分裂前DNA合成所需基因功能的鉴定。
Chromosome replication during meiosis: identification of gene functions required for premeiotic DNA synthesis.
作者信息
Roth R
出版信息
Proc Natl Acad Sci U S A. 1973 Nov;70(11):3087-91. doi: 10.1073/pnas.70.11.3087.
Abstract
Recent comparisons of chromosome replication in meiotic and mitotic cells have revealed significant differences in both the rate and pattern of DNA synthesis during the final duplication preceding meiosis. These differences suggested that unique gene functions might be required for premeiotic replication that were not necessary for replication during growth. To provide evidence for such functions, we isolated stage-specific mutants in the yeast Saccharomyces cerevisiae which permitted vegetative replication but blocked the round of replication before meiosis. The mutants synthesized carbohydrate, protein, and RNA during the expected interval of premeiotic replication, suggesting that their lesions preferentially affected synthesis of DNA. The mutations blocked meiosis, as judged by a coincident inhibition of intragenic recombination and ascospore formation. The lesions were characterized as recessive nuclear genes, and were designated mei-1, mei-2, and mei-3; complementation indicated that the relevant gene products were not identical.
摘要
近期对减数分裂细胞和有丝分裂细胞中染色体复制的比较研究表明，在减数分裂前的最后一次复制过程中，DNA合成的速率和模式存在显著差异。这些差异表明，减数分裂前的复制可能需要独特的基因功能，而在细胞生长过程中的复制则不需要这些功能。为了证明这些功能的存在，我们在酿酒酵母中分离出了阶段特异性突变体，这些突变体允许营养复制，但在减数分裂前阻止了一轮复制。在减数分裂前复制的预期时间段内，这些突变体合成了碳水化合物、蛋白质和RNA，这表明它们的损伤优先影响了DNA的合成。通过对基因内重组和子囊孢子形成的同时抑制判断，这些突变阻止了减数分裂。这些损伤被鉴定为隐性核基因，分别命名为mei-1、mei-2和mei-3；互补分析表明相关的基因产物并不相同。
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