van Baalen C A, Klein M R, Huisman R C, Dings M E, Kerkhof Garde S R, Geretti A M, Gruters R, van Els C A, Miedema F, Osterhaus A D
Institute of Virology, Erasmus University, Rotterdam, The Netherlands.
J Gen Virol. 1996 Aug;77 ( Pt 8):1659-65. doi: 10.1099/0022-1317-77-8-1659.
Human immunodeficiency virus type 1 (HIV-1) Gag-specific cytotoxic T lymphocyte (CTL) responses were studied in seven seropositive long-term asymptomatic individuals (CDC A1) with stable CD4 counts for more than 8 years. Using a set of partially overlapping peptides covering the whole Gag, five 15-20-mer peptides were found to contain CTL epitopes. Further characterization of these epitopes revealed a new HLA-A25-restricted CTL epitope in p24, p24(203-212) ETINEEAAEW. This region of Gag is highly conserved in clades B and D of HIV-1. Naturally occurring amino acid sequences, containing p24(203)D (consensus HIV-1 clades A, C, F, G and H) or p24(204)I (HIV-2ROD) were not recognized by CTL recognizing the index peptide. No virus variants with mutations in this sequence were found in peripheral blood mononuclear cells from the HIV-1-infected individual concerned during the 8 year observation period, indicating that the virus had not escaped from the observed CTL response.
对7名血清反应阳性的长期无症状个体（疾病控制与预防中心A1类）进行了研究，这些个体的CD4细胞计数稳定超过8年，研究了其1型人类免疫缺陷病毒（HIV-1）Gag特异性细胞毒性T淋巴细胞（CTL）反应。使用一组覆盖整个Gag的部分重叠肽段，发现5个15 - 20聚体肽段含有CTL表位。对这些表位的进一步表征揭示了p24中一个新的HLA - A25限制性CTL表位，即p24(203 - 212)ETINEEAAEW。Gag的这一区域在HIV-1的B和D亚型中高度保守。含有p24(203)D（HIV-1 A、C、F、G和H亚型的共有序列）或p24(204)I（HIV-2 ROD）的天然氨基酸序列不能被识别索引肽段的CTL识别。在8年观察期内，在所研究的HIV-1感染个体的外周血单个核细胞中未发现该序列发生突变的病毒变体，这表明病毒未逃脱所观察到的CTL反应。
