尖锐湿疣患者血清及对照组血清中针对11型人乳头瘤病毒样颗粒的抗体。

Antibodies to human papillomavirus type 11 virus-like particles in sera of patients with genital warts and in control groups.

作者信息

Eisemann C, Fisher S G, Gross G, Müller M, Gissmann L

机构信息

Deutsches Krebsforschungszentrum, Forschungsschwerpunkt Angewandte Tumorvirologie, Heidelberg, Germany.

出版信息

J Gen Virol. 1996 Aug;77 ( Pt 8):1799-803. doi: 10.1099/0022-1317-77-8-1799.

DOI:10.1099/0022-1317-77-8-1799

PMID:8760429

Abstract

We analysed by ELISA a total of 478 human sera for the presence of antibodies to HPV-11 virus-like particles. The sera were obtained from patients with current genital warts (group CO), from males attending the hospital for fertility disorders (group MA), from blood donors (group BD) and from patients hospitalized for reasons unrelated to HPV infections (group HO). Antibody prevalence was higher in male patients of group CO (23.0%) as compared to males of groups MA (3.2%; P < 0.0001), HO (5.3%; P = 0.01) and BD (16.7%; NS). In addition, there was a significant difference in antibody titre between the males of group CO compared to group MA. Within the whole sample the absorbance of sera from females was higher than in specimens from males (P < 0.0001). A small subset of the sera was also tested by radioimmunoprecipitation assay (RIPA). There was good agreement between the data obtained by ELISA and RIPA.

摘要

我们通过酶联免疫吸附测定（ELISA）分析了总共478份人血清，以检测抗人乳头瘤病毒11型（HPV - 11）病毒样颗粒抗体的存在情况。这些血清取自患有现发性尖锐湿疣的患者（CO组）、因生育障碍到医院就诊的男性（MA组）、献血者（BD组）以及因与HPV感染无关的原因住院的患者（HO组）。与MA组男性（3.2%；P < 0.0001）、HO组男性（5.3%；P = 0.01）和BD组男性（16.7%；无显著差异）相比，CO组男性患者的抗体阳性率更高（23.0%）。此外，CO组男性与MA组男性之间的抗体滴度存在显著差异。在整个样本中，女性血清的吸光度高于男性样本（P < 0.0001）。还通过放射免疫沉淀测定（RIPA）对一小部分血清进行了检测。ELISA和RIPA所获数据之间具有良好的一致性。