Guillo L A, Beylot B, Vigny P, Spassky A
Departmento de Bioquimica, Universidade de Sao Paulo, Brazil.
Photochem Photobiol. 1996 Aug;64(2):349-55. doi: 10.1111/j.1751-1097.1996.tb02470.x.
The present report provides evidence that thymine dimerization can be UVA photosensitized at a tetranucleotide, 5'-TATT-3', by a 7-methyl-pyrido(3,4-c)psoralen monoadduct in DNA. The efficiency of the photoprocess depends on the tetranucleotide flanking sequences. These results demonstrate that one DNA lesion can originate the contiguous formation of a second type of lesion and emphasize the sequence-specific response to interaction of drugs with DNA. Results are related to the sensitivity of DNA to 1,10-phenanthroline-cuprous ion complex nucleolytic activity and discussed in terms of the major role of local deformability of DNA in interaction with ligands.
本报告提供了证据，表明在DNA中，7-甲基-吡啶并(3,4-c)补骨脂素单加合物可使四核苷酸5'-TATT-3'处的胸腺嘧啶二聚化发生UVA光敏化。光过程的效率取决于四核苷酸侧翼序列。这些结果表明，一种DNA损伤可引发第二种损伤的连续形成，并强调了药物与DNA相互作用的序列特异性反应。结果与DNA对1,10-菲咯啉-亚铜离子络合物核酸酶活性的敏感性相关，并根据DNA局部可变形性在与配体相互作用中的主要作用进行了讨论。
