肺炎链球菌3型特异性合酶（Cap3B）在大肠杆菌和不同血清型的肺炎球菌菌株中指导3型多糖的生物合成。
Type 3-specific synthase of Streptococcus pneumoniae (Cap3B) directs type 3 polysaccharide biosynthesis in Escherichia coli and in pneumococcal strains of different serotypes.
作者信息
Arrecubieta C, López R, García E
机构信息
Centro de Investigaciones Biológicas, Cousejo Superior de Investigaciones Científicas, Madrid, Spain.
出版信息
J Exp Med. 1996 Aug 1;184(2):449-55. doi: 10.1084/jem.184.2.449.
Abstract
The cap3B gene, which is involved in the formation of the capsule of Streptococcus pneumoniae type 3, encodes a 49-kD protein that has been identified as a polysaccharide synthase. Escherichia coli cells harboring the recombinant plasmid pTBP3 (cap3B) produced pneumococcal type 3 polysaccharide, as demonstrated by immunological tests. Biochemical and cell fractionation analyses revealed that this polysaccharide had a high molecular mass and was localized in substantial amounts in the periplasmic space of E. coli. Unencapsulated (S-2), laboratory pneumococcal strains synthesized type 3 polysaccharide by transformation with plasmid pLSE3B harboring cap3B. In addition, encapsulated pneumococci of types 1, 2, 5, or 8 transformed with pLSE3B can direct the synthesis of pneumococcal type 3 polysaccharide, leading to the formation of strains that display binary type of capsule.
摘要
参与3型肺炎链球菌荚膜形成的cap3B基因编码一种49-kD蛋白，该蛋白已被鉴定为一种多糖合酶。携带重组质粒pTBP3（cap3B）的大肠杆菌细胞产生了3型肺炎球菌多糖，免疫试验证明了这一点。生化和细胞分级分析表明，这种多糖具有高分子量，并且大量存在于大肠杆菌的周质空间中。无荚膜（S-2）的实验室肺炎球菌菌株通过用携带cap3B的质粒pLSE3B转化来合成3型多糖。此外，用pLSE3B转化的1、2、5或8型有荚膜肺炎球菌可以指导3型肺炎球菌多糖的合成，从而导致形成具有二元荚膜类型的菌株。
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