Cella M, Scheidegger D, Palmer-Lehmann K, Lane P, Lanzavecchia A, Alber G
Basel Institute for Immunology, Switzerland.
J Exp Med. 1996 Aug 1;184(2):747-52. doi: 10.1084/jem.184.2.747.
We investigated the possibility that T helper cells might enhance the stimulatory function of dendritic cells (DCs). We found that ligation of CD40 by CD40L triggers the production of extremely high levels of bioactive IL-12. Other stimuli such as microbial agents, TNF-alpha or LPS are much less effective or not at all. In addition, CD40L is the most potent stimulus in upregulating the expression of ICAM-1, CD80, and CD86 molecules on DCs. These effects of CD40 ligation result in an increased capacity of DCs to trigger proliferative responses and IFN-gamma production by T cells. These findings reveal a new role for CD40-CD40L interaction in regulating DC function and are relevant to design therapeutic strategies using cultured DCs.
我们研究了辅助性T细胞可能增强树突状细胞（DCs）刺激功能的可能性。我们发现，CD40L与CD40的结合会触发产生极高水平的生物活性白细胞介素-12。其他刺激物，如微生物制剂、肿瘤坏死因子-α或脂多糖，效果要差得多或根本无效。此外，CD40L是上调DCs上ICAM-1、CD80和CD86分子表达的最有效刺激物。CD40结合的这些效应导致DCs触发T细胞增殖反应和产生干扰素-γ的能力增强。这些发现揭示了CD40-CD40L相互作用在调节DC功能中的新作用，并且与设计使用培养DCs的治疗策略相关。
