Giebel J, de Souza P, Rune G M
Department of Anatomy, Ernst Moritz Arndt University Greifswald, Germany.
Tissue Cell. 1996 Aug;28(4):379-85. doi: 10.1016/s0040-8166(96)80024-3.
Integrins were localized immunohistochemically in marmoset ovaries (Callithrix jacchus) of defined cycle stages. With monoclonal antibodies against beta 1, alpha 2, alpha 3, and alpha 6 integrin subunits, immunoreactivity was predominantly found in ovaries of the follicular phase. In the luteal phase, non-luteal cells, e.g. fibroblasts or endothelial cells expressed beta 1, alpha 2, or alpha 6 integrins. Immunostaining for the beta 1 subunit was strongest in granulosa cells of all growing follicles. Weak immunoreactivity was found in granulosa cells of atretic follicles, theca and interstitial cells. With the alpha 2 antibody, binding was evident in granulosa cells of many, but not of all, primordial and primary follicles. Expression of alpha 2 was also found in luteinized granulosa cells of tertiary follicles. In growing follicles, immunoreactivity of alpha 3 was restricted to granulosa cells of early secondary follicles. Within the epithelium, staining was almost exclusively found in granulosa cells bordering on the basal granulosa cell layer. In advanced stages of atresia, granulosa cells of antral follicles expressed the alpha 3 subunit. Integrin alpha 6 expression was evident in granulosa cells of all growing follicles but was absent during advanced stages of degeneration. The specific expression patterns of integrins suggest that these matrix receptors play a regulatory role during cyclic events in primate ovaries.
整合素通过免疫组织化学方法定位在特定周期阶段的狨猴卵巢（绢毛猴）中。使用针对β1、α2、α3和α6整合素亚基的单克隆抗体，免疫反应主要出现在卵泡期的卵巢中。在黄体期，非黄体细胞，如成纤维细胞或内皮细胞表达β1、α2或α6整合素。β1亚基的免疫染色在所有生长卵泡的颗粒细胞中最强。在闭锁卵泡的颗粒细胞、卵泡膜细胞和间质细胞中发现弱免疫反应。使用α2抗体时，许多但并非所有原始卵泡和初级卵泡的颗粒细胞中有明显的结合。在三级卵泡的黄体化颗粒细胞中也发现了α2的表达。在生长卵泡中，α3的免疫反应仅限于早期次级卵泡的颗粒细胞。在上皮内，染色几乎仅见于与基底颗粒细胞层相邻的颗粒细胞。在闭锁的晚期，窦状卵泡的颗粒细胞表达α3亚基。整合素α6的表达在所有生长卵泡的颗粒细胞中明显，但在退化的晚期阶段不存在。整合素的特定表达模式表明这些基质受体在灵长类卵巢的周期性事件中起调节作用。
