Wehrenberg U, Rune G M
Department of Cell Biology, E.M.A.-University, Friedrich Loeffler Strasse 23c, D-17487 Greifswald, Germany.
Mol Hum Reprod. 2000 Jun;6(6):504-9. doi: 10.1093/molehr/6.6.504.
Large non-luteinized follicles of the marmoset monkey were cultured for up to 96 h in the presence of substances that are known to induce luteinization, i.e. LH, transforming growth factor (TGF)-beta and cyclic AMP. The state of the basal lamina, and the expression of connexin-43, alpha(2) integrin subunit and TGF-beta receptor type II (TbetaR-II) were chosen as parameters to judge the progress of luteinization. Antral follicles, cultured for 1 h, were not luteinized, as shown by an intact basal lamina, strong immunoreactivity of connexin-43 in granulosa cells, and no expression of TbetaR-II in the theca layer. After 12 h, most follicles showed a dissolution of the basal lamina, a faint reactivity of connexin-43, high expression of TbetaR-II in theca- and outer granulosa cells and high expression of alpha(2) integrin subunit in granulosa cells bordering at the basement membrane; all of which indicate luteinization. After 96 h of culture, luteal structures (e.g. corpora lutea accessoria) had developed. This was true for both non-stimulated and stimulated follicles. Our results strongly suggest that antral follicles luteinize spontaneously. The decisive determinant appears to be the follicular stage.
将狨猴的大型未黄体化卵泡在已知可诱导黄体化的物质（即促黄体生成素、转化生长因子（TGF）-β和环磷酸腺苷）存在的情况下培养长达96小时。选择基膜状态、连接蛋白-43、α(2)整合素亚基和II型TGF-β受体（TβR-II）的表达作为判断黄体化进程的参数。培养1小时的窦状卵泡未发生黄体化，表现为基膜完整、颗粒细胞中连接蛋白-43免疫反应性强以及卵泡膜层中无TβR-II表达。12小时后，大多数卵泡显示基膜溶解、连接蛋白-43反应微弱、卵泡膜和外层颗粒细胞中TβR-II高表达以及与基底膜相邻的颗粒细胞中α(2)整合素亚基高表达；所有这些都表明发生了黄体化。培养96小时后，黄体结构（如副黄体）已经发育。未刺激和刺激的卵泡都是如此。我们的结果强烈表明窦状卵泡会自发黄体化。决定性因素似乎是卵泡阶段。
