Blanco-Rodríguez J, Martínez-García C
Department of Cell Biology, School of Medicine, Valladolid University, Spain.
Tissue Cell. 1996 Aug;28(4):387-99. doi: 10.1016/s0040-8166(96)80025-5.
The analysis of the morphological response of the seminiferous epithelium to hormone suppression after hypophysectomy has been widely used in the study of spermatogenesis. Unlike hypophysectomy, which causes a sharp fall in gonadotropin levels, oestradiol treatment, although mimicking its effects, leads to a partial and progressive hormone suppression. We have chosen this model in order to clarify further the early morphological response of the seminiferous epithelium to hormone suppression. For this purpose, adult male rats, injected daily from 1-35 days with 50 micrograms oestradiol benzoate, were analyzed. Our observations showed that cell sloughing was the main cause of germ cell depletion in the oestradiol treated testis. After 17-18 days treatment, massive sloughing affected nearly all the germinal cells, but the correct synchronization of mitoses, meiosis and spermatid differentiation along the 14 stages of spermatogenesis was always well kept. Quantitative analysis showed that spermatogenic wave was well preserved in the oestradiol-treated animals and that sloughing was a stage-independent phenomenon.
垂体切除术后生精上皮对激素抑制的形态学反应分析已广泛应用于精子发生的研究。与导致促性腺激素水平急剧下降的垂体切除术不同，雌二醇治疗虽然模拟了其作用，但会导致部分且渐进性的激素抑制。我们选择这个模型是为了进一步阐明生精上皮对激素抑制的早期形态学反应。为此，对从第1天到第35天每天注射50微克苯甲酸雌二醇的成年雄性大鼠进行了分析。我们的观察结果表明，细胞脱落是雌二醇处理的睾丸中生精细胞耗竭的主要原因。治疗17 - 18天后，大量脱落影响了几乎所有的生殖细胞，但在精子发生的14个阶段中，有丝分裂、减数分裂和精子细胞分化的正确同步性始终保持良好。定量分析表明，在雌二醇处理的动物中精子发生波保存良好，并且脱落是一种与阶段无关的现象。
