使用组蛋白作为促进剂来提高逆转录病毒基因转移的效率。
The use of histone as a facilitator to improve the efficiency of retroviral gene transfer.
作者信息
Singh D, Rigby P W
机构信息
Division of Eukaryotic Molecular Genetics, MRC National Institute for Medical Research, London, UK.
出版信息
Nucleic Acids Res. 1996 Aug 1;24(15):3113-4. doi: 10.1093/nar/24.15.3113.
Abstract
Vectors based on murine C-type retroviruses are commonly used in biology. The efficiency of viral infection is normally increased by a facilitator, for example polybrene, DEAE-dextran or a liposome. The receptor for ecotropic viruses is a transporter for basic amino acids; we therefore explored the use of a highly basic protein, histone type IIA, as a facilitator. We show in several cell types that histone is as efficient as the other agents tested, and in some cases more so. This readily available reagent is thus likely to be useful in the wide range of studies that employ retroviral vectors.
摘要
基于鼠类C型逆转录病毒的载体在生物学中常用。病毒感染效率通常由一种促进剂提高，例如聚凝胺、二乙氨基乙基葡聚糖或脂质体。嗜亲性病毒的受体是碱性氨基酸的转运体；因此，我们探索使用一种高度碱性的蛋白质，即组蛋白IIA型，作为促进剂。我们在几种细胞类型中表明，组蛋白与其他测试试剂一样有效，在某些情况下甚至更有效。因此，这种易于获得的试剂可能在广泛使用逆转录病毒载体的研究中有用。
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本文引用的文献
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