Trinel P A, Cantelli C, Bernigaud A, Jouault T, Poulain D
Unité 42, Institut National de la Santé et de la Recherche Médicale, Villeneuve d'Ascq, France.
Microbiology (Reading). 1996 Aug;142 ( Pt 8):2263-70. doi: 10.1099/13500872-142-8-2263.
A monoclonal antibody specific for beta-1,2-linked oligomannosides was used to study the association of these residues with Candida albicans mannan and phospholipomannan (PLM) in relation to growth conditions and in mannan mutant strains. Double immunofluorescence assays performed on cells grown under standard conditions indicated a highly heterogeneous cell surface expression of these epitopes in comparison with the homogeneous expression of alpha-linked oligomannosidic epitopes. Growth in the presence of tunicamycin, which inhibits mannan N-glycosylation, resulted in an absence of beta-1,2-oligomannosidic epitopes on the cell surface, although PLM synthesis still occurred as shown by autoradiography. Similarly, growth in acidic conditions, which inhibits the incorporation of beta-1,2-oligomannosides in mannan, resulted in an absence of beta-1,2-oligomannosidic epitopes at the cell surface, although they still associated with PLM as shown by Western blotting. Western blots of C. albicans mutant strains with reduced amounts or an absence of phosphorus and acid-labile beta-1,2-oligomannosides in their mannan confirmed that the association of beta-1,2-linked oligomannosides with mannan and with PLM involves different mannosylation processes.
一种对β-1,2-连接的低聚甘露糖特异的单克隆抗体被用于研究这些残基与白色念珠菌甘露聚糖和磷脂甘露聚糖（PLM）的关联，这与生长条件以及甘露聚糖突变株有关。对在标准条件下生长的细胞进行的双重免疫荧光分析表明，与α-连接的低聚甘露糖表位的均匀表达相比，这些表位在细胞表面的表达高度异质。在衣霉素存在下生长，衣霉素会抑制甘露聚糖的N-糖基化，导致细胞表面不存在β-1,2-低聚甘露糖表位，尽管放射自显影显示PLM合成仍在发生。同样，在酸性条件下生长，酸性条件会抑制β-1,2-低聚甘露糖掺入甘露聚糖，导致细胞表面不存在β-1,2-低聚甘露糖表位，尽管蛋白质印迹显示它们仍与PLM相关。对甘露聚糖中磷和酸不稳定的β-1,2-低聚甘露糖含量减少或缺失的白色念珠菌突变株进行的蛋白质印迹证实，β-1,2-连接的低聚甘露糖与甘露聚糖和PLM的关联涉及不同的甘露糖基化过程。
