Cantelli C, Trinel P A, Bernigaud A, Jouault T, Polonelli L, Poulain D
Unité 42, Institut National de la Santé et de la Recherche Médicale, Domaine du CERTIA, Villeneuve d'Ascq, France.
Microbiology (Reading). 1995 Oct;141 ( Pt 10):2693-7. doi: 10.1099/13500872-141-10-2693.
The distribution of beta-1,2-linked oligomannosides among glycoconjugates of various Candida species was investigated by Western blotting, using monoclonal and polyclonal antibodies which react with these epitopes. Expression of beta-1,2-linked oligomannosidic epitopes on a 14-18 kDa polydisperse antigen nonreactive with concanavalin A (ConA), previously identified as a C. albicans serotype A phospholipomannan (PLM), appeared to be restricted to C. albicans serotypes A and B (including var. C. stellatoidea types I and II) and C. tropicalis. In C. albicans, beta-1,2-linked oligomannosidic epitopes also appeared to be slightly associated with high molecular mass (> 100 kDa) polydisperse ConA-reactive mannoproteins. For all the other Candida strains investigated, belonging to the species C. parapsilosis, C. krusei, C. glabrata and C. robusta (S. cerevisiae), beta-1,2-linked oligomannosidic epitopes were found to be present in association with medium molecular mass (18-100 kDa) and high molecular mass ConA-reactive mannoproteins, giving reproducible labelling profiles that varied between species.
利用与这些表位反应的单克隆抗体和多克隆抗体，通过蛋白质免疫印迹法研究了β-1,2-连接的寡甘露糖在各种念珠菌属糖缀合物中的分布情况。β-1,2-连接的寡甘露糖表位在一种与伴刀豆球蛋白A（ConA）无反应的14 - 18 kDa多分散抗原上的表达，该抗原先前被鉴定为白色念珠菌A血清型磷脂甘露聚糖（PLM），似乎仅限于白色念珠菌A和B血清型（包括星状念珠菌I和II型变种）以及热带念珠菌。在白色念珠菌中，β-1,2-连接的寡甘露糖表位似乎也与高分子量（> 100 kDa）的多分散ConA反应性甘露糖蛋白有轻微关联。对于所研究的所有其他念珠菌菌株，属于近平滑念珠菌、克鲁斯念珠菌、光滑念珠菌和粗壮念珠菌（酿酒酵母），发现β-1,2-连接的寡甘露糖表位与中等分子量（18 - 100 kDa）和高分子量的ConA反应性甘露糖蛋白相关联，产生了在不同物种间有所不同的可重复标记图谱。
