泰国分离的霍乱弧菌O139亚型分型中脉冲场凝胶电泳和核糖体分型的比较

Comparison of pulsed-field gel electrophoresis and ribotyping for subtyping of Vibrio cholerae O139 isolated in Thailand.

作者信息

Dalsgaard A, Skov M N, Serichantalergs O, Echeverria P

机构信息

Department of Veterinary Microbiology, Royal Veterinary and Agricultural University, Frederiksberg C, Denmark.

出版信息

Epidemiol Infect. 1996 Aug;117(1):51-8. doi: 10.1017/s0950268800001126.

DOI:10.1017/s0950268800001126

PMID:8760950

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2271672/

Abstract

Pulsed-field gel electrophoresis (PFGE) of Cpo I-digested genomic DNA and ribotyping (Bgl I) were applied to 60 Vibrio cholerae strains including 48 V. cholerae O139 from Thailand to compare their value in differentiating strains of the present V. cholerae O139 epidemic. PFGE patterns were divided into groups A and B representing five and four subtypes, respectively, while ribotyping showed four different patterns. PFGE group B subtypes were only presented among O139 isolates from Thailand, whereas four O139 strains from Bangladesh and India showed identical PFGE group A subtypes observed in O139 isolates from Thailand. Two nontoxigenic O139 isolates from Thailand showed different and unique PFGE types as did five V. cholerae non-O139 isolates containing a gene virulence complex found in V. cholerae O139. These results indicate that PFGE (Cpo I) can resolve recent evolutionary divergence within V. cholerae O139 and offers a useful supplementary tool for following the progressing V. cholerae O139 epidemic.

摘要

对60株霍乱弧菌进行了用Cpo I消化基因组DNA的脉冲场凝胶电泳（PFGE）和核糖体分型（Bgl I），其中包括48株来自泰国的霍乱弧菌O139，以比较它们在区分当前霍乱弧菌O139流行菌株方面的价值。PFGE图谱分为A组和B组，分别代表五个和四个亚型，而核糖体分型显示出四种不同的图谱。PFGE B组亚型仅出现在来自泰国的O139分离株中，而来自孟加拉国和印度的四株O139菌株显示出与在泰国的O139分离株中观察到的相同的PFGE A组亚型。来自泰国的两株非产毒性O139分离株显示出不同且独特的PFGE类型，含有在霍乱弧菌O139中发现的基因毒力复合体的五株霍乱弧菌非O139分离株也是如此。这些结果表明，PFGE（Cpo I）可以解析霍乱弧菌O139内最近的进化分歧，并为追踪霍乱弧菌O139的流行进展提供了一个有用的补充工具。