Draoui M, Hida T, Jakowlew S, Birrer M, Zia F, Moody T W
Dept. Microbiology, George Washington Univ. Med., Ctr., Washington, DC 20037, USA.
Life Sci. 1996;59(4):307-13. doi: 10.1016/0024-3205(96)00299-8.
The effects of PACAP on c-fos mRNA using small cell lung cancer (SCLC) cell was investigated. PACAP-27 (100 nM) increased c-fos mRNA 5-fold using NCI-N417 cells. The increase was concentration dependent with 0.1 nM PACAP-27 half maximally increasing c-fos mRNA. Also the increase in c-fos mRNA caused by PACAP was time dependent; being maximal after 1 hour and returning to basal values after 4 hours. PACAP-38 but not PACAP(28-38) increased c-fos mRNA. One uM PACAP(6-38), a PACAP receptor antagonist, inhibited the increase in c-fos mRNA caused by 1 nM PACAP. These data indicate that PACAP stimulates nuclear oncogene expression in SCLC cells.
研究了垂体腺苷酸环化酶激活肽（PACAP）对小细胞肺癌（SCLC）细胞中c-fos信使核糖核酸（mRNA）的影响。使用NCI-N417细胞时，PACAP-27（100纳摩尔）使c-fos mRNA增加了5倍。这种增加呈浓度依赖性，0.1纳摩尔的PACAP-27可使c-fos mRNA增加到最大值的一半。此外，PACAP引起的c-fos mRNA增加也呈时间依赖性；1小时后达到最大值，4小时后恢复到基础值。PACAP-38能增加c-fos mRNA，但PACAP(28 - 38)则不能。1微摩尔的PACAP(6 - 38)，一种PACAP受体拮抗剂，可抑制1纳摩尔PACAP引起的c-fos mRNA增加。这些数据表明，PACAP可刺激SCLC细胞中的核癌基因表达。
