Heim J M, Singh S, Gerzer R
Abteilung für Klinische Pharmakologie, Klinikum Innenstadt der Universität, München, Germany.
Life Sci. 1996;59(4):PL61-8. doi: 10.1016/0024-3205(96)00306-2.
Cloned ANF-sensitive guanylyl cyclase (GC-A) and ANF-sensitive guanylyl cyclase from adrenal cortex differ in their sensitivity to the ANF analogs atriopeptin 1 and urodilatin. To test the hypothesis that these differences are due to different glycosylation, we investigated the effect of the N-glycosylation inhibitor tunicamycin on GC-A. Tunicamycin altered the response of GC-A to atriopeptin 1 and urodilatin, whereas the sensitivity to ANF remained unchanged. These data indicate that agonist specificities of different ANF-sensitive guanylyl cyclases are influenced by carbohydrate moieties.
克隆的心房钠尿肽（ANF）敏感型鸟苷酸环化酶（GC-A）与肾上腺皮质的ANF敏感型鸟苷酸环化酶对ANF类似物心钠素1和尿舒张素的敏感性不同。为了验证这些差异是由不同的糖基化作用所致这一假说，我们研究了N-糖基化抑制剂衣霉素对GC-A的作用。衣霉素改变了GC-A对心钠素1和尿舒张素的反应，而对ANF的敏感性保持不变。这些数据表明，不同的ANF敏感型鸟苷酸环化酶的激动剂特异性受碳水化合物部分的影响。
