Li Chen, Chen Xu, Kou Lei, Hu Bi, Zhu Li-Ping, Fan Yan-Rong, Wu Zhi-Wei, Wang Jian-Jun, Xu Gen-Xing
Department of Biological Science and Technology and State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093;
Exp Ther Med. 2010 Jan;1(1):129-135. doi: 10.3892/etm_00000022. Epub 2010 Jan 1.
Bifidobacterium longum (B. longum) as a delivery system for endostatin was shown to have definite antitumor effects. Moreover, it was found that the enrichment of selenium was able to enhance the immunity of mice. In order to further evaluate the safety and efficacy of B. longum carrying pBV22210-endostatin (B. longum-En) enriched with selenium (Se-B. longum-En), we determined the biochemical characteristics of Se-B. longum-En. We then investigated its effect on macrophage activity, as well as its inhibitory effect on the multiplication of pathogenic bacteria in vitro and the antitumor effects on murine hepatic (H22) tumor-bearing mice. The results showed that Se-B. longum-En exhibited similar biochemical characteristics to that of wild-type B. longum, i.e., Se-B. longum-En strongly enhanced macrophage phagocytosis in rats and inhibited the growth of pathogenic bacteria. In addition, Se-B. longum-En showed a definite inhibitory effect of tumor growth when H22 tumor-bearing mice were fed through oral or tail vein delivery. These results suggested that Se-B. longum is able to retain the advantages of wild-type B. longum and be used as a novel gene delivery system for liver cancer gene therapy.
长双歧杆菌（B. longum）作为内皮抑素的递送系统显示出明确的抗肿瘤作用。此外，发现硒的富集能够增强小鼠的免疫力。为了进一步评估携带pBV22210-内皮抑素的长双歧杆菌（B. longum-En）富集硒（Se-B. longum-En）的安全性和有效性，我们测定了Se-B. longum-En的生化特性。然后我们研究了其对巨噬细胞活性的影响，以及其在体外对病原菌增殖的抑制作用和对荷鼠肝癌（H22）小鼠的抗肿瘤作用。结果表明，Se-B. longum-En表现出与野生型长双歧杆菌相似的生化特性，即Se-B. longum-En强烈增强大鼠巨噬细胞吞噬作用并抑制病原菌生长。此外，当通过口服或尾静脉给药喂养荷H22肿瘤小鼠时，Se-B. longum-En对肿瘤生长显示出明确的抑制作用。这些结果表明，Se-B. longum能够保留野生型长双歧杆菌的优势，并可作为肝癌基因治疗的新型基因递送系统。
