Wall J D, Rapp-Giles B J, Rousset M
Biochemistry Department, University of Missouri--Columbia 65211.
J Bacteriol. 1993 Jul;175(13):4121-8. doi: 10.1128/jb.175.13.4121-4128.1993.
A 2.3-kb plasmid present in about 20 copies per genome was identified in extracts of Desulfovibrio desulfuricans G100A and designated pBG1. It appears to be unable to replicate in Escherichia coli. Although composite plasmids of pBG1 inserted into pTZ18U are stable in E. coli, few if any pBG1-specific transcripts are detectable. The plasmid sequence reveals several features typical of the origin of replication of non-ColE1 enterobacterial plasmids as well as several potential open reading frames. This small replicon has been shown to support the replication of recombinant plasmids in D. desulfuricans G100A and Desulfovibrio fructosovorans. A conjugable shuttle vector has been constructed.
在脱硫脱硫弧菌G100A提取物中鉴定出一种每个基因组约有20个拷贝的2.3 kb质粒，命名为pBG1。它似乎无法在大肠杆菌中复制。尽管插入pTZ18U的pBG1复合质粒在大肠杆菌中是稳定的，但几乎检测不到pBG1特异性转录本。该质粒序列揭示了非ColE1肠杆菌质粒复制起点的几个典型特征以及几个潜在的开放阅读框。这个小复制子已被证明能支持重组质粒在脱硫脱硫弧菌G100A和果糖脱硫弧菌中的复制。已构建了一种可接合的穿梭载体。
