Cutrera R A, Saboureau M, Pévet P
URA-CNRS 1332, Neurobiologie des Fonctions Rythmiques et Saisonnières, Université Louis Pasteur, Strasbourg, France.
Neurosci Lett. 1996 May 24;210(1):1-4. doi: 10.1016/0304-3940(96)12655-0.
The present results show that under constant darkness the endogenous circadian pacemaker located in the suprachiasmatic nuclei can be affected by administration of 8-hydroxy-2-[di-n-propylamino] tetralin (8-OH-DPAT), a well known 5-HT1A/5-HT7 receptor agonist. A single i.p. injection (0.1 ml) with 8-OH-DPAT (5 mg/kg) induced significant phase-advances of hamster locomotor activity at circadian time (CT) 6 and 8 and a significant phase-delay at CT11. Saline injections by themselves induced a significant phase-advance at CT10-11. The dose-response curve for 8-OH-DPAT showed a maximal phase-shifting effect for doses of at least 2.5 mg/kg at CT8. Thus, in golden hamsters. (1) 8-OH-DPAT has a chronobiological effect with sensitivity depending upon the circadian time of injection, and (2) a single saline injection is able to induce regular phase-advances at the end of the subjective day (CT10-11).
目前的结果表明，在持续黑暗条件下，位于视交叉上核的内源性昼夜节律起搏器会受到8-羟基-2-[二正丙基氨基]四氢萘（8-OH-DPAT，一种著名的5-HT1A/5-HT7受体激动剂）给药的影响。腹腔注射（0.1毫升）一次8-OH-DPAT（5毫克/千克）在昼夜时间（CT）6和8时诱导仓鼠运动活动出现显著的相位提前，在CT11时出现显著的相位延迟。单独注射生理盐水在CT10-11时诱导出现显著的相位提前。8-OH-DPAT的剂量-反应曲线显示，在CT8时，至少2.5毫克/千克的剂量具有最大的相位移动效应。因此，在金黄仓鼠中，（1）8-OH-DPAT具有时间生物学效应，其敏感性取决于注射的昼夜时间，并且（2）单次注射生理盐水能够在主观日结束时（CT10-11）诱导规律的相位提前。
