Lorca M, Schenone H, del C Contreras M, García A, Bahamonde M I, Correa V, Valdés J, Rojas A, Jofré A
Departamento de Parasitología, Universidad de Chile, Santiago.
Bol Chil Parasitol. 1995 Jul-Dec;50(3-4):87-91.
The present study deals with the evaluation of the effectiveness of the measures employed in the control of Triatoma infestans, the vector of Trypanosoma cruzi infection (Chagas' disease), in the IV region of Chile through a serological follow up program. For this reason, a serological survey has been carried out in 2,783 children, from 0 to 10 years, who live in rural high endemic areas of the region. The sera were tested using an indirect hemagglutination test (IHAT) and ELISA techniques with results agreeing in 99.9% of the samples. In children with positive serology xenodiagnosis was performed and blood samples from the corresponding mothers were submitted to serology for Chagas' disease. These additional tests should allow to distinguish between congenital infection and vectorial transmission. It was observed that vectorial transmission of T. cruzi was interrupted in some localities where entomological control has been applied for 10 years, or lowered in those which have been submitted to desinsectations in the last 5 years. On the other hand, a relatively high prevalence of the infection has been detected in some localities. These results suggest an apparent increase of vectorial transmission, indicating the need of readjusting the dwellings insecticide sprayings activities to improve the measures against the vector.
本研究通过一项血清学随访计划,对智利第四大区采用的控制克鲁斯锥虫(查加斯病)传播媒介——侵扰锥猎蝽的措施的有效性进行评估。因此,对该地区农村高流行区的2783名0至10岁儿童进行了血清学调查。血清样本采用间接血凝试验(IHAT)和酶联免疫吸附测定(ELISA)技术检测,结果在99.9%的样本中一致。对血清学呈阳性的儿童进行了活体接种诊断,并将相应母亲的血样送去做查加斯病血清学检测。这些额外检测应能区分先天性感染和媒介传播。据观察,在一些实施了10年昆虫学控制措施的地区,克鲁斯锥虫的媒介传播已被阻断,而在过去5年进行过灭虫处理的地区,媒介传播有所减少。另一方面,在一些地区检测到相对较高的感染率。这些结果表明媒介传播明显增加,这表明需要重新调整住宅杀虫剂喷洒活动,以改进针对该传播媒介的措施。