Clarke S C, McIntyre M
Department of Microbiology, Wexham Park Hospital, Slough, Berkshire.
J Clin Pathol. 1996 Jun;49(6):511-2. doi: 10.1136/jcp.49.6.511.
Cyclospora cayetanensis is a cause of prolonged diarrhoea, mainly in travellers. Laboratory diagnosis may be achieved by a number of methods such as the staining of faecal smears by the modified Ziehl-Neelsen (ZN) technique. Safer methods using this technique have been described for the staining of acid fast bacilli and cryptosporidia by replacing the phenol content of the carbol fuschin stain with various concentrated detergents. In this report the technique was modified slightly using a non-concentrated detergent and applied to the staining of oocysts of C cayetanensis. It was found that oocysts of C cayetanensis do not stain using the modified detergent ZN method when compared with similar preparations containing oocysts of Cryptosporidium spp.
卡耶塔环孢子球虫是导致长期腹泻的病因之一，主要见于旅行者。实验室诊断可通过多种方法实现，比如用改良齐-尼（ZN）技术对粪便涂片进行染色。已经有更安全的方法使用该技术来对抗酸杆菌和隐孢子虫进行染色，即通过用各种浓缩洗涤剂替代石炭酸复红染液中的苯酚成分。在本报告中，使用一种非浓缩洗涤剂对该技术进行了略微改良，并将其应用于卡耶塔环孢子球虫卵囊的染色。结果发现，与含有隐孢子虫属卵囊的类似标本相比，卡耶塔环孢子球虫卵囊不能用改良洗涤剂ZN法染色。
