脑膜败血金黄杆菌的核糖体分型：作为一种流行病学工具的应用及其与血清型的相关性。

Quilici M L, Bizet C

Collection de l'Institut Pasteur, Paris.

Res Microbiol. 1996 Jun;147(5):415-25. doi: 10.1016/0923-2508(96)84716-4.

Chryseobacterium meningosepticum (basonym, Flavobacterium meningosepticum King 1959) is associated with neonatal meningitis and is isolated from normal and immunocompromised adults. AAF-labelled Escherichia coli 16 + 23S rRNA was used as a probe for ribotype analysis of 92 clinical isolates from tracheal exsudate, blood culture, cerebrospinal fluid, urine and pus. The 92 isolates belonged to the 15 described serovars of C. meningosepticum, and included 21 strains isolated during an outbreak in an intensive care unit, all belonging to serovar G. Three restriction endonucleases, EcoRI, HindIII and PstI, were selected for use in ribotyping after preliminary experiments. Epidemiologically unrelated isolates were discriminated by ribotyping and could be classified into 48 ribotypes according to the hybridization banding patterns obtained after restriction with the three enzymes. Strains which were not discriminated by combined ribotype analysis belonged to the same serovar, and were of identical geographic origin. In one case, analysis with an additional enzyme, PvuII, was necessary for separating strains from two different serovars. However, three strains from different serovars (two isolated from the same place and one elsewhere within eight years) showed the same combined ribotype. Analysis of the rRNA gene patterns revealed 6 different patterns for clinical isolates of the outbreak, suggesting unrelated sources of infection. In three patients, isolation of C. meningosepticum with different combined ribotypes suggested superinfection. Ribotyping enabled differentiation between isolates belonging to the same serovar as well as between isolates of different serovars and provided a useful molecular epidemiological tool for the study of C. meningosepticum. Combined ribotype analysis with several restriction endonucleases increased the discriminating power of the method. However, there was only a partial correlation between serovars and the extent of DNA relatedness.

脑膜败血金黄杆菌（曾用名，脑膜败血黄杆菌，金氏1959年命名）与新生儿脑膜炎有关，可从正常成年人和免疫功能低下的成年人中分离得到。用AAF标记的大肠杆菌16 + 23S rRNA作为探针，对92株来自气管渗出液、血培养、脑脊液、尿液和脓液的临床分离株进行核糖体分型分析。这92株分离株属于已描述的15种脑膜败血金黄杆菌血清型，其中包括在一个重症监护病房暴发期间分离出的21株菌株，均属于血清型G。经过初步实验，选择了三种限制性内切酶EcoRI、HindIII和PstI用于核糖体分型。通过核糖体分型可区分流行病学上不相关的分离株，根据用这三种酶酶切后获得的杂交带型可将其分为48种核糖体类型。通过联合核糖体分型分析无法区分的菌株属于同一血清型，且地理来源相同。在一个案例中，需要用另外一种酶PvuII进行分析才能区分来自两种不同血清型的菌株。然而，来自不同血清型的三株菌株（其中两株在八年内从同一地点分离得到，另一株在其他地方分离得到）显示出相同的联合核糖体类型。对rRNA基因模式的分析揭示了暴发的临床分离株有6种不同模式，提示感染源不相关。在三名患者中，分离出具有不同联合核糖体类型的脑膜败血金黄杆菌提示存在重复感染。核糖体分型能够区分属于同一血清型的分离株以及不同血清型的分离株，为脑膜败血金黄杆菌的研究提供了一种有用的分子流行病学工具。用几种限制性内切酶进行联合核糖体分型分析提高了该方法的鉴别能力。然而，血清型与DNA相关性程度之间仅存在部分相关性。