Dean G A, Reubel G H, Moore P F, Pedersen N C
Department of Medicine, School of Veterinary Medicine, University of California, Davis 95616, USA.
J Virol. 1996 Aug;70(8):5165-9. doi: 10.1128/JVI.70.8.5165-5169.1996.
Feline immunodeficiency virus (FIV) is similar to human immunodeficiency virus type 1 virologically and induces a clinical syndrome in cats comparable to human immunodeficiency virus type 1 syndrome in humans. To determine the lymphoid target cells of FIV, populations of CD4+ lymphocytes, CD8+ lymphocytes, and CD21+ lymphocytes (B cells) were enriched to more than 96.5% purity and then analyzed for FIV provirus by semiquantitative DNA amplification. We found FIV provirus in CD4+, CD8+, and B lymphocytes. In cats infected for <4 months, proviral burden was greatest in CD4+ cells, followed by B cells and then by CD8+ cells. In cats infected for more than 5 years, proviral burden was greatest in B cells, followed by CD4+ cells and then by CD8+ cells. The total proviral burden was > 1 log10 higher in acutely infected cats than in chronically infected cats, primarily because of a higher level of CD4+ infection in the acutely infected cats. A comparison of proviral loads in mesenteric lymph node and peripheral blood mononuclear cells in acutely or chronically infected cats revealed no significant difference. A kinetics study of FIV infection demonstrated that all lymphocyte subpopulations were infected by 4 weeks postinoculation. Virus was isolated from CD4+, CD8+, and B cells in vitro, and reverse transcriptase PCR demonstrated that all subsets contained viral RNA in vivo and therefore are productive reservoirs for FIV.
猫免疫缺陷病毒（FIV）在病毒学上与人类免疫缺陷病毒1型相似，并在猫中引发一种临床综合征，类似于人类免疫缺陷病毒1型在人类中引发的综合征。为了确定FIV的淋巴靶细胞，将CD4 +淋巴细胞、CD8 +淋巴细胞和CD21 +淋巴细胞（B细胞）群体富集至纯度超过96.5%，然后通过半定量DNA扩增分析FIV前病毒。我们在CD4 +、CD8 +和B淋巴细胞中发现了FIV前病毒。在感染时间小于4个月的猫中，前病毒负荷在CD4 +细胞中最高，其次是B细胞，然后是CD8 +细胞。在感染超过5年的猫中，前病毒负荷在B细胞中最高，其次是CD4 +细胞，然后是CD8 +细胞。急性感染猫的总前病毒负荷比慢性感染猫高> 1 log10，主要是因为急性感染猫中CD4 +感染水平更高。对急性或慢性感染猫的肠系膜淋巴结和外周血单个核细胞中的前病毒载量进行比较，未发现显著差异。一项FIV感染的动力学研究表明，接种后4周时所有淋巴细胞亚群均被感染。体外从CD4 +、CD8 +和B细胞中分离出病毒，逆转录酶PCR表明所有亚群在体内均含有病毒RNA，因此是FIV的有效储存库。
