Voss T G, Fermin C D, Levy J A, Vigh S, Choi B, Garry R F
Department of Microbiology and Immunology, Tulane University School of Medicine, New Orleans, Louisiana 70112, USA.
J Virol. 1996 Aug;70(8):5447-54. doi: 10.1128/JVI.70.8.5447-5454.1996.
Increases in intracellular concentrations of potassium ([K+]i) and sodium ([Na+]i) occur concomitantly with cytopathic effects induced in a CD4+ T-lymphoblastoid cell line acutely infected by human immunodeficiency virus (HIV). This [K+]i increase was greater in cells infected by cytopathic HIV strains than in cells infected by less cytopathic strains. T cells persistently infected by HIV had an increased [K+]i but displayed an [Na+]i similar to that of mock-infected cells. HIV induced increases in [K+]i and [Na+]i after cytopathic infection of human peripheral blood mononuclear cells, but the magnitude of the Na+ changes did not correlate with the extent of the cytopathic effect. Enhanced movement of cations may osmotically drive water entry, resulting in balloon degeneration and lysis of HIV-infected cells. These observations offer potential approaches for antiviral therapies.
在人免疫缺陷病毒（HIV）急性感染的CD4 + T淋巴母细胞系中，细胞内钾离子浓度（[K + ]i）和钠离子浓度（[Na + ]i）的升高与细胞病变效应同时出现。与感染细胞病变程度较轻的HIV毒株的细胞相比，感染细胞病变性HIV毒株的细胞中[K + ]i升高幅度更大。持续感染HIV的T细胞[K + ]i升高，但[Na + ]i与模拟感染细胞相似。HIV感染人外周血单个核细胞导致细胞病变后，[K + ]i和[Na + ]i升高，但Na +变化的幅度与细胞病变效应的程度无关。阳离子运动增强可能通过渗透作用驱动水进入，导致HIV感染细胞出现气球样变性和裂解。这些观察结果为抗病毒治疗提供了潜在的方法。
