General Pathology Division, Triangulo Mineiro Federal University, Rua Frei Paulino 30, Bairro Abadia, CEP: 38025-180, Uberaba, Minas Gerais, Brazil.
Virchows Arch. 2012 Oct;461(4):449-55. doi: 10.1007/s00428-012-1297-9. Epub 2012 Aug 16.
Acquired immunodeficiency syndrome (AIDS) is characterized by decreased immunity, making a patient more susceptible to opportunistic infections which can have cutaneous manifestations. The aim of this study was to evaluate the local immunity of the skin through morphological and immunohistochemical analysis. Skin samples of 52 women, 27 without AIDS and 25 with AIDS, autopsied in an academic referral hospital in Brazil were evaluated. The autopsy reports and medical records were reviewed, and histochemical Hematoxylin-eosin, Picrosirius red, and Verhoeff stains as well as morphometric (Image J and KS-300 Kontron-Zeiss) and immunohistochemical (S-100 and anti-IgA) analyses of the skin were performed. Women with AIDS presented a thinner epidermis than women without AIDS (33.33 [12.00-317.66] vs 67.42 [12.00-530.02] μm; p < 0.001), with a lower number of epithelial cell layers (4.00 [2.00-11.00] vs 4.00 [2.00-16.00]; p < 0.001), a smaller cell diameter (12.92 [6.00-28.87] vs 24.32 [6.00-33.12] μm; p < 0.001), and a lower number of Langerhans cells (LC) (12.58 [0.00-81.74] vs 31.44 [0.00-169.77] LC/mm(2); p < 0.001). The dermis contained more collagen fibers (8.20 % [2.40-19.40] vs 6.30 % [0.40-13.90]; p < 0.001). Some of these parameters were negatively correlated with viral load and positively correlated with the number of CD4+ T-lymphocytes. We conclude that a decrease of the local skin immunity in women with AIDS may contribute to the development of skin lesions.
获得性免疫缺陷综合征 (AIDS) 的特征是免疫力下降，使患者更容易受到机会性感染，这些感染可能会有皮肤表现。本研究旨在通过形态学和免疫组织化学分析来评估皮肤的局部免疫。对巴西一家学术转诊医院进行尸检的 52 名女性的皮肤样本进行了评估，其中 27 名女性没有艾滋病，25 名女性患有艾滋病。回顾了尸检报告和病历，并对皮肤进行了组织化学苏木精-伊红、苦味酸-天狼星红和Verhoeff 染色以及形态计量学（Image J 和 KS-300 Kontron-Zeiss）和免疫组织化学（S-100 和抗 IgA）分析。患有艾滋病的女性的表皮比没有艾滋病的女性薄（33.33 [12.00-317.66] 与 67.42 [12.00-530.02] μm；p<0.001），上皮细胞层数较少（4.00 [2.00-11.00] 与 4.00 [2.00-16.00]；p<0.001），细胞直径较小（12.92 [6.00-28.87] 与 24.32 [6.00-33.12] μm；p<0.001），朗格汉斯细胞（LC）较少（12.58 [0.00-81.74] 与 31.44 [0.00-169.77] LC/mm(2)；p<0.001）。真皮含有更多的胶原纤维（8.20% [2.40-19.40] 与 6.30% [0.40-13.90]；p<0.001）。其中一些参数与病毒载量呈负相关，与 CD4+ T 淋巴细胞数量呈正相关。我们得出结论，艾滋病女性的局部皮肤免疫下降可能导致皮肤损伤的发生。
