Nichol P F, Chang J Y, Johnson E M, Olivo P D
Department of Molecular Biology and Pharmacology, Washington UniversitySchool of Medicine, St. Louis, Missouri 63110, USA.
J Virol. 1996 Aug;70(8):5476-86. doi: 10.1128/JVI.70.8.5476-5486.1996.
Herpes simplex virus establishes a latent infection in peripheral neurons. We examined viral gene expression in rat peripheral neurons in vitro and determined that viral gene expression is attenuated and delayed in these neurons compared with that in Vero cells. In addition, using pharmacologic and genetic blocks to viral DNA synthesis, we found that viral alpha and beta gene expression was upregulated by viral DNA synthesis. Although maximal gene expression in neurons requires viral DNA synthetic activity, activation of viral gene expression was seen even in the presence of herpes simplex virus DNA polymerase inhibitors, but not in the absence of the origin-binding protein. Initiation of viral DNA synthesis is apparently a key regulatory event in the balance between the lytic and latent pathways in peripheral neurons.
单纯疱疹病毒在周围神经元中建立潜伏感染。我们在体外检测了大鼠周围神经元中的病毒基因表达，并确定与非洲绿猴肾细胞中的病毒基因表达相比，这些神经元中的病毒基因表达减弱且延迟。此外，通过对病毒DNA合成进行药理学和遗传学阻断，我们发现病毒α和β基因表达因病毒DNA合成而上调。虽然神经元中的最大基因表达需要病毒DNA合成活性，但即使在存在单纯疱疹病毒DNA聚合酶抑制剂的情况下也能观察到病毒基因表达的激活，而在没有起始结合蛋白的情况下则不能。病毒DNA合成的起始显然是周围神经元裂解和潜伏途径平衡中的一个关键调节事件。
