Gama Sosa M A, Hall J C, Ruprecht R M
Dana-Farber Cancer Institute, Department of Pathology, Boston, MA.
Biochem Biophys Res Commun. 1988 Oct 14;156(1):417-23. doi: 10.1016/s0006-291x(88)80857-x.
We have examined the S1 nuclease sensitivity of supercoiled plasmids harboring the Moloney Murine Leukemia Virus (MoMuLV) long terminal repeat (LTR). S1 sensitivity was found within the LTR enhancer direct repeats. Transformation of E. coli DH5 cells with a construct containing most of the MoMuLV LTR yielded the precise deletion of one direct repeat and loss of S1 sensitivity. The dependence of S1 sensitivity on the presence of both direct repeats, together with the exact excision of one direct repeat by E. coli, suggests the presence of slipped DNA within the enhancer. Such structures may represent targets for effector proteins which mediate vital functions during viral propagation.
我们检测了携带莫洛尼鼠白血病病毒（MoMuLV）长末端重复序列（LTR）的超螺旋质粒的S1核酸酶敏感性。在LTR增强子直接重复序列内发现了S1敏感性。用包含大部分MoMuLV LTR的构建体转化大肠杆菌DH5细胞，导致一个直接重复序列的精确缺失和S1敏感性的丧失。S1敏感性对两个直接重复序列存在的依赖性，以及大肠杆菌对一个直接重复序列的精确切除，表明增强子内存在滑动DNA。这种结构可能代表了在病毒传播过程中介导重要功能的效应蛋白的作用靶点。
