Morrione A, Valentinis B, Li S, Ooi J Y, Margolis B, Baserga R
Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA.
Cancer Res. 1996 Jul 15;56(14):3165-7.
Using the yeast two-hybrid system, we have isolated a new substrate of the insulin-like growth factor I receptor (IGF-IR), identified as Grb10, a member of the family of SH2 domain proteins. With the help of several mutants of the IGF-IR, we have mapped the binding site of Grb10 between amino acids 1229 and 1245 of the receptor, a sequence that is dispensable for the mitogenic activity of the IGF-IR. Grb10 coprecipitates with the IGF-IR in cell lysates and is probably involved in the regulation of its activity.
利用酵母双杂交系统，我们分离出胰岛素样生长因子I受体（IGF-IR）的一种新底物，鉴定为Grb10，它是SH2结构域蛋白家族的成员。借助IGF-IR的几种突变体，我们已将Grb10的结合位点定位在受体的1229至1245位氨基酸之间，该序列对于IGF-IR的促有丝分裂活性是可有可无的。Grb10在细胞裂解物中与IGF-IR共沉淀，可能参与其活性调节。
