Monda M, Papa A, Brizzi G, DeLuca B
Department of Human Physiology and Integrated Biological Functions F. Bottazzi, Second University of Naples, Italy.
Am J Physiol. 1996 Jun;270(6 Pt 2):R1215-9. doi: 10.1152/ajpregu.1996.270.6.R1215.
The firing rate of the nerves innervating interscapular brown adipose tissue (IBAT), IBAT and colonic temperatures (TIBAT and TC), and O2 consumption were monitored in urethan-anesthetized male Sprague-Dawley rats. These variables were measured for 40 min before (baseline values) and 40 min after a neostigmine (5 x 10(-7) mol) or saline injection in the hippocampus. The blood level of 3,5,3'-triiodothyronine and L-thyroxine (T3 and T4) and the 5'-deiodinating activity of IBAT, liver, and kidneys were determined in other rats with neostigmine or saline injection. The results showed that neostigmine injection increased firing rate, TIBAT, TC, O2 consumption, blood level of T3, and 5'-deiodinating activity of IBAT. No change was found in the T4 level and in 5'-deiodinating activity of the liver and kidneys. These findings suggest that neostigmine injection in the hippocampus increases heat production by stimulating sympathetic nerves to IBAT and by elevating the blood level of T3.
在氨基甲酸乙酯麻醉的雄性Sprague-Dawley大鼠中，监测支配肩胛间棕色脂肪组织（IBAT）的神经放电频率、IBAT和结肠温度（TIBAT和TC）以及耗氧量。在海马体注射新斯的明（5×10⁻⁷摩尔）或生理盐水之前（基线值）和之后40分钟测量这些变量。在其他注射新斯的明或生理盐水的大鼠中，测定3,5,3'-三碘甲状腺原氨酸和L-甲状腺素（T3和T4）的血液水平以及IBAT、肝脏和肾脏的5'-脱碘活性。结果表明，注射新斯的明可增加放电频率、TIBAT、TC、耗氧量、T3血液水平以及IBAT的5'-脱碘活性。未发现T4水平以及肝脏和肾脏的5'-脱碘活性有变化。这些发现表明，海马体注射新斯的明可通过刺激支配IBAT的交感神经以及提高T3血液水平来增加产热。
