Milner P, Batten J E, Curtis M A
MRC Molecular Pathogenesis Group, Department of Oral Microbiology, St. Bartholomew's, London, UK.
FEMS Microbiol Lett. 1996 Jul 1;140(2-3):125-30. doi: 10.1016/0378-1097(96)00159-0.
The aim of this study was the development of a simple, defined medium for the growth of laboratory and clinical isolates of Porphyromonas gingivalis. A medium was designed in which the carbon and nitrogen requirements were provided by a single protein source--bovine serum albumin. High cell yields were achieved in this medium but growth was accompanied by a heavy blackening of the cells due to the deposition of metal sulfide(s), most probably iron(II) sulfide, at the cell surface. Good growth in the absence of blackening was achieved when the iron salt in the medium was substituted with alpha-ketoglutarate. The resultant alpha-ketoglutarate/BSA medium was able to support the growth of all laboratory and clinical P. gingivalis strains examined and should prove useful in the investigation of the physiology and nutritional regulation of virulence of this organism.
本研究的目的是开发一种简单、明确的培养基，用于牙龈卟啉单胞菌实验室菌株和临床分离株的培养。设计了一种培养基，其中碳源和氮源由单一蛋白质来源——牛血清白蛋白提供。在这种培养基中可获得高细胞产量，但由于金属硫化物（很可能是硫化亚铁）在细胞表面沉积，细胞生长时会伴随严重变黑。当培养基中的铁盐被α-酮戊二酸替代时，可实现无变黑情况下的良好生长。所得的α-酮戊二酸/牛血清白蛋白培养基能够支持所检测的所有牙龈卟啉单胞菌实验室菌株和临床菌株的生长，在该生物体毒力的生理学和营养调节研究中应会证明是有用的。
