Fujii S, Iwahashi H, Obuchi K, Fujii T, Komatsu Y
Department of Bioproduction Science, Chiba University, Matsudo, Japan.
FEMS Microbiol Lett. 1996 Jul 15;141(1):97-101. doi: 10.1111/j.1574-6968.1996.tb08369.x.
A barotolerant mutant of Saccharomyces cerevisiae and its parent were compared with respect to the barotolerance of each strain, intracellular content of trehalose, amounts of three major heat shock proteins (hsps), relative proportion of unsaturated and saturated fatty acids in membrane phospholipids, and membrane fluidity. Changes in the amount of trehalose, but not hsps, were associated with changes in the barotolerance of each strain during the different phases of growth and with the difference in barotolerance between the mutant and parent strain. The membrane fluidity in the case of the mutant was low, while that of the parent was high. These results suggest that accumulation of trehalose and the condition of the membrane are more important for barotolerance than accumulation of hsps.
就各菌株的耐压性、海藻糖的细胞内含量、三种主要热休克蛋白（hsps）的量、膜磷脂中不饱和脂肪酸和饱和脂肪酸的相对比例以及膜流动性而言，对酿酒酵母的耐压突变体及其亲本进行了比较。在不同生长阶段，各菌株耐压性的变化与海藻糖含量的变化相关，而非与热休克蛋白的变化相关，并且与突变体和亲本菌株之间的耐压性差异相关。突变体的膜流动性较低，而亲本的膜流动性较高。这些结果表明，海藻糖的积累和膜的状态对耐压性而言比热休克蛋白的积累更为重要。
