Blazer-Yost B L, Record R D, Oberleithner H
Biology Department, Indiana University/Purdue University, 723 West Michigan Street, Indianapolis, IN 46202, USA.
Pflugers Arch. 1996 Aug;432(4):685-91. doi: 10.1007/s004240050186.
The Madin-Darby canine kidney (MDCK) cell line forms an epithelial monolayer which expresses many of the morphological and functional properties of the renal collecting duct. The C7 subclone of the parent line forms an epithelium which expresses many of the characteristics of principal cells. The MDCK-C7 subclone forms a high-resistance epithelium that is capable of vectorial ion transport. We have found that this epithelium responds to aldosterone, antidiuretic hormone (ADH) and insulin like growth factor 1 (IGF1) with increases in amiloride-sensitive Na+ transport. The responses to aldosterone and ADH follow time-courses that are consistent with the action of these hormones in vivo. This is the first demonstration of IGF1-induced Na+ reabsorption in a mammalian model system. Interestingly, a maximal response to any one of these natriferic factors does not inhibit a subsequent response to another hormone. These studies indicate that the C7 subclone retains many of the natriferic responses of the native principal cells and is an ideal model for studying hormonal modulation of Na+ transport.
麦氏犬肾（MDCK）细胞系形成上皮单层，表达肾集合管的许多形态学和功能特性。亲代细胞系的C7亚克隆形成表达主细胞许多特征的上皮。MDCK-C7亚克隆形成能够进行向量离子转运的高电阻上皮。我们发现，这种上皮对醛固酮、抗利尿激素（ADH）和胰岛素样生长因子1（IGF1）有反应，对氨氯地平敏感的Na+转运增加。对醛固酮和ADH的反应遵循与这些激素在体内作用一致的时间进程。这是IGF1诱导哺乳动物模型系统中Na+重吸收的首次证明。有趣的是，对任何一种促钠因子的最大反应都不会抑制随后对另一种激素的反应。这些研究表明，C7亚克隆保留了许多天然主细胞的促钠反应，是研究Na+转运激素调节的理想模型。
