Kouritas V K, Ioannou M, Foroulis C N, Desimonas N, Evaggelopoulos K, Gourgoulianis K I, Molyvdas P A, Hatzoglou C
Department of Physiology, Medical School, University of Thessaly, Mezourlo, New Buildings, Larissa, Greece.
Exp Diabetes Res. 2010;2010:853176. doi: 10.1155/2010/853176. Epub 2010 Aug 12.
Insulin directly changes the sheep pleural electrophysiology. The aim of this study was to investigate whether insulin induces similar effects in human pleura, to clarify insulin receptor's involvement, and to demonstrate if glibenclamide (hypoglycemic agent) reverses this effect.
Human parietal pleural specimens were mounted in Ussing chambers. Solutions containing insulin or glibenclamide and insulin with anti-insulin antibody, anti-insulin receptor antibody, and glibenclamide were used. The transmesothelial resistance (R(TM)) was determined. Immunohistochemistry for the presence of Insulin Receptors (IRa, IRb) was also performed.
Insulin increased R(TM) within 1st min (P = .016), when added mesothelially which was inhibited by the anti-insulin and anti-insulin receptor antibodies. Glibenclamide also eliminated the insulin-induced changes. Immunohistochemistry verified the presence of IRa and IRb.
Insulin induces electrochemical changes in humans as in sheep via interaction with its receptor. This effect is abolished by glibenclamide.
胰岛素可直接改变绵羊胸膜的电生理。本研究旨在探究胰岛素在人胸膜中是否会产生类似效应，阐明胰岛素受体的作用，并验证格列本脲（一种降糖药）是否能逆转这种效应。
将人壁层胸膜标本置于尤斯灌流小室中。使用含有胰岛素或格列本脲以及胰岛素与抗胰岛素抗体、抗胰岛素受体抗体和格列本脲的溶液。测定跨间皮电阻（R(TM)）。还进行了胰岛素受体（IRa、IRb）存在情况的免疫组织化学检测。
当从间皮侧加入胰岛素时，在第1分钟内R(TM)升高（P = 0.016），而抗胰岛素和抗胰岛素受体抗体可抑制此升高。格列本脲也消除了胰岛素诱导的变化。免疫组织化学证实了IRa和IRb的存在。
胰岛素通过与其受体相互作用，在人体中诱导出与绵羊类似的电化学变化。这种效应可被格列本脲消除。
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