Bonnefoix T, Bonnefoix P, Verdiel P, Sotto J J
ID9 SOFT, Grenoble, France.
J Immunol Methods. 1996 Aug 14;194(2):113-9. doi: 10.1016/0022-1759(96)00077-4.
Limiting dilution analysis is a common technique that is used in immunology to estimate accurately the frequency of cells possessing a wide variety of functional activities such as growth, cytotoxicity and production of lymphokines. The reliability of the estimated frequency is usually checked by a standard chi-square (x2) test validating the goodness-of-fit to the single-hit Poisson model (SHPM). We present evidence that modelling limiting dilution data according to a generalized linear model offers an alternative to the standard x2 test for detecting departures from the SHPM, with a considerable increase in power compared to the x2 test.
有限稀释分析是免疫学中常用的一种技术，用于准确估计具有多种功能活性（如生长、细胞毒性和淋巴因子产生）的细胞频率。估计频率的可靠性通常通过标准卡方（x2）检验来检查，该检验用于验证与单打击泊松模型（SHPM）的拟合优度。我们提供的证据表明，根据广义线性模型对有限稀释数据进行建模，为检测偏离SHPM的情况提供了一种替代标准x2检验的方法，与x2检验相比，功效有显著提高。
