Alsip N L, Hornung J W, Saha P R, Hill J B, Asher E F
Center for Applied Microcirculatory Research, University of Louisville School of Medicine, KY, USA.
Am J Obstet Gynecol. 1996 Aug;175(2):388-95. doi: 10.1016/s0002-9378(96)70151-5.
Our purpose was to develop a method for direct measurement of rat uterine microvessels and to test their viability.
In anesthetized female Sprague-Dawley diestrus rats, one uterine hom was isolated from the body cavity with its nerve and blood supply intact. A small fiberoptic probe inserted into the lumen of the uterus served as a light source for transillumination of uterine vessels. Diameters of circumferential arterioles were observed while increasing concentrations of vasoactive agonists were suffused over the uterus.
No significant diameter changes occurred in circumferential arterioles of the control group (100-minute suffusion of Krebs solution). Dilation with papaverine (100 mumol/L) demonstrated that vessels possessed basal tone. Circumferential arterioles showed concentration-dependent constriction to phenylephrine and angiotensin II and dilation to acetylcholine and serotonin.
This uterine microcirculatory preparation provides a stable, reproducible model of a unique microcirculatory bed that responds to vasoactive agents in a manner similar to other microcirculatory beds.
我们的目的是开发一种直接测量大鼠子宫微血管的方法，并测试其活力。
在麻醉的雌性斯普拉格 - 道利动情间期大鼠中，将一侧子宫从体腔中分离出来，其神经和血液供应保持完整。插入子宫腔内的一根小型光纤探头用作子宫血管透照的光源。在向子宫灌注浓度不断增加的血管活性激动剂时，观察环行小动脉的直径。
对照组（用克雷布斯溶液灌注100分钟）的环行小动脉直径无明显变化。用罂粟碱（100μmol/L）扩张表明血管具有基础张力。环行小动脉对去氧肾上腺素和血管紧张素II呈浓度依赖性收缩，对乙酰胆碱和血清素呈扩张反应。
这种子宫微循环制备方法提供了一个稳定、可重复的独特微循环床模型，其对血管活性药物的反应方式与其他微循环床相似。
