Kajimura N, Iseki H, Tanaka R, Ohue C, Otsubo K, Gyoutoku M, Sasaki K, Akiyama Y, Yamaguchi K
Growth Factor Division, National Cancer Center Research Institute, Tokyo, Japan.
Cancer Chemother Pharmacol. 1996;38 Suppl:S48-52. doi: 10.1007/s002800051038.
Toxohormones are tumor-derived factors that induce cancer cachexia syndrome in tumor-bearing animals. Nude mice bearing tumors induced by eight human cancer cell lines with this activity were studied for cytokine production and expression of a newly identified gene, ob, which has the ability to control body weight. A melanoma cell line, SEKI, and a neuroepithelioma cell line, NAGAI, produced a large amount of the cytokine, leukemia-inhibitory factor (LIF). A uterine carcinoma cell line, Yumoto, produced a large amount of interleukin 6 (IL-6), and an oral cavity carcinoma cell line, OCC-1C, concomitantly produced LIF, IL-6, and IL-11. Reverse transcription polymerase chain reaction studies revealed that ob gene mRNA was not expressed in any of these cell lines, suggesting that the gene does not have a role as a tumor product responsible for cancer cachexia in this model. These findings suggest that in four of eight animal models in which cancer cachexia syndrome developed, LIF, IL-6, or possibly IL-11 produced by cancer cells may be toxohormones, but in the remaining four cancer cell lines the mechanism responsible for cachexia syndrome remains unknown.
毒激素是肿瘤衍生因子，可在荷瘤动物中诱发癌症恶病质综合征。对携带由具有这种活性的八种人类癌细胞系诱导的肿瘤的裸鼠进行了细胞因子产生和一种新鉴定的基因ob表达的研究，该基因具有控制体重的能力。一种黑色素瘤细胞系SEKI和一种神经上皮瘤细胞系NAGAI产生大量细胞因子，即白血病抑制因子（LIF）。一种子宫癌细胞系Yumoto产生大量白细胞介素6（IL-6），而一种口腔癌细胞系OCC-1C同时产生LIF、IL-6和IL-11。逆转录聚合酶链反应研究表明，这些细胞系中均未表达ob基因mRNA，这表明该基因在该模型中并非作为导致癌症恶病质的肿瘤产物发挥作用。这些发现表明，在八种出现癌症恶病质综合征的动物模型中的四种中，癌细胞产生的LIF、IL-6或可能的IL-11可能是毒激素，但在其余四种癌细胞系中，导致恶病质综合征的机制仍然未知。
