Oskam L, Schoone G J, Kroon C C, Lujan R, Davies J B
Royal Tropical Institute, Department of Biomedical Research, Amsterdam, The Netherlands.
Trop Med Int Health. 1996 Aug;1(4):522-7. doi: 10.1046/j.1365-3156.1996.d01-95.x.
The detection of Onchocerca volvulus infected simuliids or blackflies is routinely done by dissection and microscopic examination of individual flies, but this method is tedious and time consuming. Here we describe a method of detecting single O. volvulus infected blackflies in pools of uninfected blackflies. Using a PCR with Onchocerca specific primers it is possible to reproducibly detect one heavily infected blackfly in a pool of 80 flies, or to detect one blackfly inoculated with one microfilaria in a pool of 20 flies. With the method described large numbers of blackflies can be rapidly screened for the presence of O. volvulus infected flies.
盘尾丝虫感染蚋或黑蝇的检测通常是通过对单个蝇进行解剖和显微镜检查来完成的，但这种方法既繁琐又耗时。在此，我们描述了一种在未感染黑蝇群体中检测单个感染盘尾丝虫黑蝇的方法。使用针对盘尾丝虫的特异性引物进行聚合酶链反应（PCR），能够重复检测出80只蝇群体中的一只重度感染黑蝇，或者在20只蝇的群体中检测出一只接种有一条微丝蚴的黑蝇。通过所描述的方法，可以快速筛查大量黑蝇，以确定是否存在感染盘尾丝虫的黑蝇。
