编码一种螺旋-环-螺旋蛋白（小鼠ID1B）的cDNA的分子克隆：ID1A和ID1B基因的组织特异性表达

Molecular cloning of the cDNA encoding a helix-loop-helix protein, mouse ID1B: tissue-specific expression of ID1A and ID1B genes.

作者信息

Hernandez M C, Andres-Barquin P J, Israel M A

机构信息

Department of Neurological Surgery, School of Medicine, University of California, San Francisco 94143-0520, USA.

出版信息

Biochim Biophys Acta. 1996 Jul 31;1308(1):28-30. doi: 10.1016/0167-4781(96)00092-9.

DOI:10.1016/0167-4781(96)00092-9

PMID:8765747

Abstract

A cDNA encoding a 168 amino acid mouse ID1B helix-loop-helix protein, the longest among the ID family of proteins so far identified, was cloned and its nucleotide sequence determined. Mouse ID1B mRNA is distinguishable from the mRNA encoding ID1A at its 3' end, and the relative level of expression of these two different mRNAs is similar in most tissues, with exception of skeletal muscle and kidney. Comparison of the most carboxyl terminal predicted amino acid sequences of ID1 proteins reveals 100% identity for ID1A, but the predicted Id1B proteins of several species are different.

摘要

克隆了一个编码168个氨基酸的小鼠ID1B螺旋-环-螺旋蛋白的cDNA，它是迄今为止在ID蛋白家族中发现的最长的蛋白，并测定了其核苷酸序列。小鼠ID1B mRNA在其3'端与编码ID1A的mRNA不同，并且这两种不同mRNA的相对表达水平在大多数组织中相似，但骨骼肌和肾脏除外。ID1蛋白的最羧基末端预测氨基酸序列比较显示，ID1A的序列一致性为100%，但几个物种的预测Id1B蛋白有所不同。