Frew A J, St-Pierre J, Teran L M, Trefilieff A, Madden J, Peroni D, Bodey K M, Walls A F, Howarth P H, Carroll M P, Holgate S T
University Medicine, Southampton General Hospital, England.
J Allergy Clin Immunol. 1996 Jul;98(1):133-43. doi: 10.1016/s0091-6749(96)70235-x.
The effects of acute allergen exposure on bronchoalveolar lavage cells and mediators and mucosal inflammatory cells were evaluated in 10 subjects with atopic asthma who underwent lavage and biopsy 24 hours after segmental endobronchial allergen challenge. Increased numbers of bronchoalveolar lavage eosinophils were retrieved from the allergen-challenged sites compared with the saline-challenged sites (mean 21.4 vs 1.5 x 10(3) cells/ml; p < 0.02). Numbers of neutrophils and proportions of CD4+, CD8+, CD25+, and HLA-DR+ T cells were similar at the saline- and allergen-challenged sites. In contrast to the bronchoalveolar lavage findings, eosinophil numbers were not increased in the bronchial submucosa or epithelium. There was also no significant difference in neutrophils, mast cells, CD3+, CD4+, or CD8+ T cells in the submucosa after allergen challenge, but the number of activated (CD25+) T lymphocytes in the mucosa did increase after allergen challenge. Allergen challenge did not induce any significant change in endothelial expression of P-selectin, E-selectin, intercellular adhesion molecule-1, or vascular cell adhesion molecule-1. CD11a+ and very late antigen-4+ cell numbers were similar in the saline- and allergen-challenged sites. This study suggests that in patients with very mild asthma, local allergen challenge induces persistent bronchoalveolar lavage eosinophilia, but the recruitment process seems to have diminished or ceased by 24 hours.
在10名特应性哮喘患者中，评估了急性变应原暴露对支气管肺泡灌洗细胞、介质和黏膜炎症细胞的影响。这些患者在支气管内节段性变应原激发后24小时接受了灌洗和活检。与盐水激发部位相比，从变应原激发部位回收的支气管肺泡灌洗嗜酸性粒细胞数量增加（平均分别为21.4×10³细胞/毫升和1.5×10³细胞/毫升；p<0.02）。盐水激发部位和变应原激发部位的中性粒细胞数量以及CD4⁺、CD8⁺、CD25⁺和HLA-DR⁺T细胞比例相似。与支气管肺泡灌洗结果相反，支气管黏膜下层或上皮中的嗜酸性粒细胞数量并未增加。变应原激发后，黏膜下层的中性粒细胞、肥大细胞、CD3⁺、CD4⁺或CD8⁺T细胞也没有显著差异，但变应原激发后黏膜中活化（CD25⁺）T淋巴细胞的数量确实增加了。变应原激发未引起P选择素、E选择素、细胞间黏附分子-1或血管细胞黏附分子-1在内皮表达上的任何显著变化。盐水激发部位和变应原激发部位的CD11a⁺和极晚期抗原-4⁺细胞数量相似。这项研究表明，在非常轻度哮喘患者中，局部变应原激发可诱导持续性支气管肺泡灌洗嗜酸性粒细胞增多，但募集过程在24小时时似乎已经减弱或停止。
