Chen S, Soneji V, Webster J
Abbott Laboratories, Department 98U, Abbott Park, IL 60064, USA.
J Chromatogr A. 1996 Jul 19;739(1-2):351-7. doi: 10.1016/0021-9673(96)00215-4.
Choline is a primary degradation product of succinylcholine chloride. Determination of low concentration choline in succinylcholine chloride bulk drug and formulation is a challenge, due to the lack of sensitive detection methods. A reversed-phase separation method with postcolumn suppression conductivity detection is described for the determination of choline. Hexanesulfonic acid is employed as an ion-pair reagent in the mobile phase, which allows the accomplishment of both reversed-phase separation and a sensitive conductivity detection. Detection sensitivity is significantly enhanced by passing the mobile phase through a postcolumn cation suppressor, where hexanesulfonic acid is removed and the background conductance is reduced. This method is simple and sensitive. No sample derivatization procedure is required. The detection limit for choline is about 10 pmol.
胆碱是氯化琥珀胆碱的主要降解产物。由于缺乏灵敏的检测方法，测定氯化琥珀胆碱原料药及制剂中的低浓度胆碱具有挑战性。本文描述了一种采用柱后抑制电导检测的反相分离方法来测定胆碱。己烷磺酸用作流动相中的离子对试剂，可实现反相分离和灵敏的电导检测。通过使流动相通过柱后阳离子抑制器，去除己烷磺酸并降低背景电导，显著提高了检测灵敏度。该方法简单且灵敏。无需样品衍生化步骤。胆碱的检测限约为10皮摩尔。
