Luterotti S, Maysinger D
Department of Pharmacology and Therapeutics, McGill University, Montréal, Quebec, Canada.
J Pharm Biomed Anal. 1994 Sep;12(9):1083-90. doi: 10.1016/0731-7085(94)00050-6.
A simple and reliable method of assaying succinylcholine chloride, oxtriphylline (choline theophyllinate) and acetycholine chloride in pharmaceutical preparations, based on conversion to choline, is provided by combination of chemiluminometry of choline and dot-blot technique. Recoveries of 102 and 97% for choline chloride in Quelicin injection and Choledyl 200 tablets matrices, respectively, and of 99% for acetylcholine chloride in Miochol solution matrix could be achieved using simple choline chloride standards in phosphate buffer pH 8.6. Accordingly, matrix-matched standards were found redundant. Favourable results obtained in preparations-matched media including limits of detection of 37-39 pmol microliters-1 of choline chloride, together with accuracies of 0-2% and RSDs ranging from 4 to 7% are the further evidence of the suitability of the method.
基于转化为胆碱的原理，一种简单可靠的用于测定药物制剂中氯化琥珀胆碱、胆茶碱和氯化乙酰胆碱的方法，是通过胆碱化学发光法和斑点印迹技术相结合来实现的。在pH 8.6的磷酸盐缓冲液中使用简单的氯化胆碱标准品，在Quelicin注射液和Choledyl 200片基质中，氯化胆碱的回收率分别为102%和97%，在Miochol溶液基质中，氯化乙酰胆碱的回收率为99%。因此，发现基质匹配标准品是多余的。在制剂匹配介质中获得的良好结果，包括氯化胆碱的检测限为37 - 39 pmol μL-1，准确度为0 - 2%，相对标准偏差为4%至7%，进一步证明了该方法的适用性。
