Noguchi S, Hiratsuka M, Furukawa H, Aihara T, Kasugai T, Tamura S, Imaoka S, Koyama H, Iwanaga T
Department of Surgery, Center for Adult Diseases, Osaka, Japan.
Jpn J Cancer Res. 1996 Jun;87(6):650-4. doi: 10.1111/j.1349-7006.1996.tb00272.x.
A sensitive method for the detection of gastric cancer micrometastases in lymph nodes was developed. The method was based on amplification of keratin 19 mRNA by reverse transcriptase-polymerase chain reaction (RT-PCR). Keratin 19 RT-PCR showed that keratin 19 mRNA was expressed in all 12 gastric cancers, but not in any of 20 normal control lymph nodes, indicating that keratin 19 mRNA is a good target of RT-PCR for the detection of gastric cancer micrometastases in lymph nodes. Serial dilution studies of RNA extracted from gastric cancers against RNA extracted from control lymph nodes demonstrated that the detection sensitivity of the keratin 19 RT-PCR method was one cancer cell in 10(3)-10(5) lymph node cells. Detectability of lymph node metastases was compared between keratin 19 RT-PCR and conventional histological examination, using 100 lymph nodes obtained from 12 gastric cancer patients. Keratin 19 mRNA was detected in all of the seven lymph nodes which were histologically metastasis-positive. Of the 93 lymph nodes which were histologically metastasis-negative, 79 were found not to express keratin 19 mRNA but 14 were found to express keratin 19 mRNA, indicating that these lymph nodes contained micrometastases which could not be detected by histological examination. These results demonstrate that keratin 19 RT-PCR is a more sensitive method than histological examination for the detection of gastric micrometastases in lymph nodes.
开发了一种检测淋巴结中胃癌微转移的灵敏方法。该方法基于通过逆转录聚合酶链反应（RT-PCR）扩增角蛋白19 mRNA。角蛋白19 RT-PCR显示，12例胃癌中均表达角蛋白19 mRNA，但20例正常对照淋巴结中均未表达，这表明角蛋白19 mRNA是RT-PCR检测淋巴结中胃癌微转移的良好靶点。对从胃癌中提取的RNA与从对照淋巴结中提取的RNA进行系列稀释研究表明，角蛋白19 RT-PCR方法的检测灵敏度为10(3)-10(5)个淋巴结细胞中有一个癌细胞。使用从12例胃癌患者获得的100个淋巴结，比较了角蛋白19 RT-PCR与传统组织学检查对淋巴结转移的可检测性。在所有7个组织学转移阳性的淋巴结中均检测到角蛋白19 mRNA。在93个组织学转移阴性的淋巴结中，79个未检测到角蛋白19 mRNA表达，但14个检测到角蛋白19 mRNA表达，这表明这些淋巴结含有组织学检查无法检测到的微转移。这些结果表明，角蛋白19 RT-PCR在检测淋巴结中胃癌微转移方面比组织学检查更灵敏。
