Sharma V, Srinivas V R, Surolia A
Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India.
FEBS Lett. 1996 Jul 8;389(3):289-92. doi: 10.1016/0014-5793(96)00613-8.
We report cloning of the DNA encoding winged bean basic agglutinin (WBA I). Using oligonucleotide primers corresponding to N- and C-termini of the mature lectin, the complete coding sequence for WBA I could be amplified from genomic DNA. DNA sequence determination by the chain termination method revealed the absence of any intervening sequences in the gene. The DNA deduced amino acid sequence of WBA I displayed some differences with its primary structure established previously by chemical means. Comparison of the sequence of WBA I with that of other legume lectins highlighted several interesting features, including the existence of the largest specificity determining loop which might account for its oligosaccharide-binding specificity and the presence of an additional N-glycosylation site. These data also throw some light on the relationship between the primary structure of the protein and its probable mode of dimerization.
我们报道了编码四棱豆碱性凝集素（WBA I）的DNA的克隆。使用与成熟凝集素的N端和C端相对应的寡核苷酸引物，可以从基因组DNA中扩增出WBA I的完整编码序列。通过链终止法进行的DNA序列测定表明该基因中不存在任何间隔序列。WBA I的DNA推导氨基酸序列与其先前通过化学方法确定的一级结构存在一些差异。将WBA I的序列与其他豆科植物凝集素的序列进行比较，突出了几个有趣的特征，包括存在可能解释其寡糖结合特异性的最大特异性决定环以及存在一个额外的N-糖基化位点。这些数据也为蛋白质的一级结构与其可能的二聚化模式之间的关系提供了一些线索。
