Kirkeby S, Singha N C, Surolia A
Department of Oral Function and Physiology, Faculty of Health Sciences, University of Copenhagen, Denmark. Svend
Histochem Cell Biol. 1997 Jan;107(1):31-7. doi: 10.1007/s004180050086.
The winged bean (Psophocarpus tetragonolobus) agglutinin (total lectin) and its basic (WBA I) and acidic isoform (WBA II) were used to analyze capillaries in sections from human muscle. The microvessels were clearly labeled after incubation with the lectins in both normal muscle and in old muscles with age-related type II atrophy or muscle fiber grouping. Muscle fibers, nerves, and connective tissue remained unstained. The total lectin detected muscle capillaries from all blood group AB0 individuals. The isoform WBA I reacted only with blood vessels in blood group A and B individuals, while the blood vessels in blood group 0 individuals were demonstrated with WBA II. WBA I staining was inhibited by p-nitrophenyl alpha-galactopyranoside and N-acetylgalactosamine, whereas 2'-fucosyllactose and preincubation with an antibody against type-1 chain H abolished capillary staining with WBA II. The study demonstrates the usefulness of WBA as a marker of capillaries in human muscle.
四棱豆（Psophocarpus tetragonolobus）凝集素（总凝集素）及其碱性同工型（WBA I）和酸性同工型（WBA II）被用于分析人类肌肉切片中的毛细血管。在正常肌肉以及伴有与年龄相关的II型萎缩或肌纤维群组化的老龄肌肉中，用这些凝集素孵育后，微血管被清晰标记。肌纤维、神经和结缔组织仍未染色。总凝集素能检测所有ABO血型个体的肌肉毛细血管。同工型WBA I仅与A血型和B血型个体的血管发生反应，而O血型个体的血管则由WBA II显示。WBA I染色被对硝基苯基α - 吡喃半乳糖苷和N - 乙酰半乳糖胺抑制，而2'-岩藻糖基乳糖以及用抗1型链H抗体预孵育可消除WBA II对毛细血管的染色。该研究证明了WBA作为人类肌肉中毛细血管标志物的有用性。
