Yamamoto Y, Kubota T, Atoji Y, Suzuki Y
Laboratory of Veterinary Anatomy, Department of Veterinary Science, Faculty of Agriculture, Gifu University, 501-11 Gifu, Japan.
Cell Tissue Res. 1996 Jul;285(1):109-17. doi: 10.1007/s004410050626.
The morphology of the perilobular connective tissue of the deep proventricular gland of the chicken was examined, with special reference to myofibroblasts. Observations were made after immunohistochemical staining for alpha-vascular smooth muscle actin, gamma-enteric smooth muscle actin, and desmin, as well as by scanning and transmission electron microscopy. A perilobular sheath containing multiple layers of flat cells and an elastic network was observed around each lobule by scanning electron microscopy. The flat cells included myofibroblasts, fibroblasts, and a few smooth muscle cells. Myofibroblasts were immunopositive for alpha-vascular smooth muscle actin but immunonegative for gamma-enteric smooth muscle actin and desmin. Smooth muscle cells were immunopositive for gamma-enteric smooth muscle actin and desmin, as well as for alpha-vascular smooth muscle actin. Fibroblasts failed to bind any of the antibodies used in this study. The ultrastructural characteristics of myofibroblasts were intermediate to those of smooth muscle cells and fibroblasts. Small numbers of thin filaments and a few thick filaments were present in the myofibroblast cytoplasm. Dense bodies and caveolae were seldom observed. Cytoplasmic processes of the myofibroblasts extended along the bases of the lobules, and desmosomes and gap junctions interconnected the processes. Myofibroblasts in the sheaths may collaborate with fibers in the elastic network to prevent collapse of the glandular lobules.
对鸡深前胃腺小叶周围结缔组织的形态进行了检查，特别关注了肌成纤维细胞。在对α-血管平滑肌肌动蛋白、γ-肠平滑肌肌动蛋白和结蛋白进行免疫组织化学染色后，以及通过扫描电子显微镜和透射电子显微镜进行观察。通过扫描电子显微镜观察到每个小叶周围有一个包含多层扁平细胞和弹性网络的小叶鞘。扁平细胞包括肌成纤维细胞、成纤维细胞和一些平滑肌细胞。肌成纤维细胞对α-血管平滑肌肌动蛋白呈免疫阳性，但对γ-肠平滑肌肌动蛋白和结蛋白呈免疫阴性。平滑肌细胞对γ-肠平滑肌肌动蛋白、结蛋白以及α-血管平滑肌肌动蛋白均呈免疫阳性。成纤维细胞未能与本研究中使用的任何抗体结合。肌成纤维细胞的超微结构特征介于平滑肌细胞和成纤维细胞之间。肌成纤维细胞胞质中存在少量细丝和一些粗丝。很少观察到致密体和小窝。肌成纤维细胞的胞质突起沿小叶基部延伸，桥粒和缝隙连接使这些突起相互连接。鞘中的肌成纤维细胞可能与弹性网络中的纤维协同作用，以防止腺小叶塌陷。
