Yamamoto Y, Kubota T, Atoji Y, Suzuki Y
Laboratory of Veterinary Anatomy, Faculty of Agriculture, Gifu University, Japan.
J Anat. 1996 Dec;189 ( Pt 3)(Pt 3):623-30.
Immunoreactivity specific for alpha-vascular smooth muscle actin (ASMA) was examined in the enteric smooth muscle cells along the entire length of the gastrointestinal tract of the chicken. Specificity for gamma-smooth muscle actin (GSMA) and desmin was also examined. All smooth muscle layers, i.e. the muscularis mucosae, and the circular and longitudinal muscle layers, showed immunoreactivity specific for GSMA and desmin throughout the gastrointestinal tract whereas immunoreactivity for ASMA differed between regions and muscle layers. In the oesophagus and crop, immunoreactivity for ASMA was observed in the muscularis mucosae and the inner and outer muscle layers, together with staining for GSMA and desmin. In the proventriculus, immunoreactivity for ASMA was observed in all smooth muscle cells in the inner layer of the muscularis mucosae and the longitudinal muscle layer. In the outer layer of the muscularis mucosae, immunoreactivity for ASMA on smooth muscle cells was observed on the luminal side and decreased in the serosal direction. In the intermediate muscles, immunoreactivity for ASMA was observed in the luminal portion, the intensity of staining decreasing gradually in the serosal direction. In contrast to the intermediate muscles, the latter muscles were negative for ASMA. In the pyloric region, the outer part was weakly immunopositive, while the inner part was intensely positive. In the small and large intestines, the muscularis mucosae and the longitudinal muscle layer were positive for ASMA. The outer part of the circular muscle layer was immunonegative for ASMA whereas the inner part was positive. The complex structure and contractile functions of each organ and muscle layers may be related to the difference patterns of expression of ASMA molecules in the smooth muscle cells.
在鸡胃肠道的整个长度范围内，对肠平滑肌细胞中α - 血管平滑肌肌动蛋白（ASMA）的免疫反应性进行了检测。还检测了γ - 平滑肌肌动蛋白（GSMA）和结蛋白的特异性。所有平滑肌层，即黏膜肌层、环形肌层和纵行肌层，在整个胃肠道中均显示出对GSMA和结蛋白的特异性免疫反应性，而ASMA的免疫反应性在不同区域和肌层之间存在差异。在食管和嗉囊中，在黏膜肌层以及内、外肌层中观察到了ASMA的免疫反应性，同时还有GSMA和结蛋白的染色。在前胃中，在黏膜肌层内层和纵行肌层中的所有平滑肌细胞中均观察到了ASMA的免疫反应性。在黏膜肌层外层，平滑肌细胞上ASMA的免疫反应性在腔面观察到，并在浆膜方向减弱。在中间肌中，在腔面部分观察到了ASMA的免疫反应性，染色强度在浆膜方向逐渐降低。与中间肌相反，后段肌肉对ASMA呈阴性。在幽门区域，外部弱阳性，而内部强阳性。在小肠和大肠中，黏膜肌层和纵行肌层对ASMA呈阳性。环形肌层的外部对ASMA免疫阴性，而内部呈阳性。每个器官和肌层的复杂结构和收缩功能可能与平滑肌细胞中ASMA分子表达的不同模式有关。
