Scapol L, Marchi E, Viscomi G C
Alfa Wassermann S.p.A., Bologna, Italy.
J Chromatogr A. 1996 May 31;735(1-2):367-74. doi: 10.1016/0021-9673(95)00813-6.
Capillary electrophoresis at constant voltage with the addition of triethylamine as electrolyte to a running buffer containing borate using fused-silica capillaries permits the complete resolution in less than 30 min of 11 standard heparin and 8 standard dermatan sulfate disaccharides, which represent degradation products of heparin and dermatan sulfate by specific lyases. Triethylamine influences the migration time of disaccharides by reducing both their electrophoretic mobility towards the anode and the electroosmotic flow towards the cathode. A modulated combination of these effects together with borate-disaccharide complex formation is responsible for separation, especially in the case of isomers which differ in the position of the sulfate groups. The addition of acetonitrile did not introduce any favourable effect in the separation of disaccharide mixtures. Under these conditions different dermatan sulfates were analysed to assess the source of the preparations.
在使用熔融石英毛细管的含硼酸盐运行缓冲液中添加三乙胺作为电解质，进行恒压毛细管电泳，可在不到30分钟的时间内完全分离11种标准肝素二糖和8种标准硫酸皮肤素二糖，这些二糖代表肝素和硫酸皮肤素被特定裂解酶降解的产物。三乙胺通过降低二糖向阳极的电泳迁移率和向阴极的电渗流来影响其迁移时间。这些效应的调节组合以及硼酸盐 - 二糖复合物的形成导致了分离，特别是对于硫酸根基团位置不同的异构体。添加乙腈对二糖混合物的分离没有产生任何有利影响。在这些条件下，对不同的硫酸皮肤素进行了分析，以评估制剂的来源。
