Gotti R, Cavrini V, Andrisano V, Mascellani G
Dipartimento di Scienze Farmaceutiche, Università di Bologna, Italy.
J Chromatogr A. 1998 Jul 24;814(1-2):205-11. doi: 10.1016/s0021-9673(98)00434-8.
Dermatan sulfate (DS), a complex, polydispersed, sulfate polysaccharide was investigated as a useful chiral selector in capillary electrophoresis for the enantioresolution of a variety of drugs. Analysis was carried out in a fused-silica capillary column of 48.5 cm length (40 cm to detector window) x 50 microns I.D., and the separation buffer consisted of citric acid-Tris containing DS; the applied voltage was 15 kV and the detection wavelength was 220 nm. The effects of buffer pH, the dermatan concentration and run temperature on the enantioseparation and migration were examined. The method was applied to the enantioresolution of a representative set of twenty basic drugs. At all pH values used (3.0, 4.5 and 6.5) the addition of DS resulted in an increased migration time due to analyte-DS interaction. Using DS concentration of 2% (w/W), at pH 4.5, enantiomeric separations could be obtained for more than 50% of the examined drugs; resorcinic drugs; resorcinic moiety was found to be a very favourable structural feature for obtaining high enantioresolution values.
硫酸皮肤素（DS）是一种复杂的、多分散的硫酸化多糖，被研究作为毛细管电泳中用于多种药物对映体拆分的有效手性选择剂。分析在一根长度为48.5厘米（至检测窗口40厘米）、内径为50微米的熔融石英毛细管柱中进行，分离缓冲液由含有DS的柠檬酸 - 三羟甲基氨基甲烷组成；施加电压为15 kV，检测波长为220纳米。考察了缓冲液pH值、硫酸皮肤素浓度和运行温度对对映体分离和迁移的影响。该方法应用于一组二十种代表性碱性药物的对映体拆分。在所有使用的pH值（3.0、4.5和6.5）下，由于分析物与DS的相互作用，添加DS导致迁移时间增加。在pH 4.5时，使用2%（w/W）的DS浓度，超过50%的受试药物可实现对映体分离；间苯二酚类药物；发现间苯二酚部分是获得高对映体拆分值的非常有利的结构特征。
