Andreeva E R, Khubulova I M, Serebriakov V N, Orekhov A N
Tsitologiia. 1996;38(3):319-24.
The rate of gap junction communication has been investigated in the primary culture of highly differentiated mesenchymal cells (subendothelial smooth muscle cells isolated from grossly normal and atherosclerotic areas of human aorta), and in poorly differentiated cells of mesenchymal origin (adult human skin fibroblasts as well as skin fibroblasts and aortic smooth muscle cells derived from human fetus). In cell cultures isolated from grossly normal and atherosclerotic aorta the number of cells coupled via gap junction increased with cell density and reached the plateau at a cell density of 50-70 cells/mm2. In cultures of normal aortic cells the number of coupled cells was significantly higher than in cultures of atherosclerotic cells. Gap junctional communication between cells loaded with lipid inclusions was lower that that between cells free of excess of intracellular lipids. In cultures of human skin fibroblasts the rate of intercellular communication was comparable to that in cultures of atherosclerotic cells. There was practically no cell-to-cell communication in cultures of fetal cells. It is hypothesized that the reduced gap junctional communication in atherosclerotic human aorta is associated with alterations in the degree of smooth muscle cell differentiation.
已在高度分化的间充质细胞（从人主动脉大体正常和动脉粥样硬化区域分离的内皮下平滑肌细胞）的原代培养物以及间充质来源的低分化细胞（成人皮肤成纤维细胞以及源自人胎儿的皮肤成纤维细胞和主动脉平滑肌细胞）中研究了间隙连接通讯的速率。在从大体正常和动脉粥样硬化主动脉分离的细胞培养物中，通过间隙连接耦合的细胞数量随细胞密度增加而增加，并在细胞密度为50 - 70个细胞/mm²时达到平台期。在正常主动脉细胞培养物中，耦合细胞的数量显著高于动脉粥样硬化细胞培养物。含有脂质包涵体的细胞之间的间隙连接通讯低于不含细胞内脂质过量的细胞之间的间隙连接通讯。在人皮肤成纤维细胞培养物中，细胞间通讯速率与动脉粥样硬化细胞培养物中的相当。在胎儿细胞培养物中几乎没有细胞间通讯。据推测，人动脉粥样硬化主动脉中间隙连接通讯的减少与平滑肌细胞分化程度的改变有关。
